Quantification of nisin concentration from fluorescence-based antimicrobial activity assay using Bayesian calibration.

Autor: Steier V; Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Jülich, Germany.; Institute of Biotechnology, RWTH Aachen University, Aachen, Germany., Osthege M; Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Jülich, Germany., Helleckes LM; Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Jülich, Germany.; Institute of Biotechnology, RWTH Aachen University, Aachen, Germany., Siska M; Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Jülich, Germany.; Computational Systems Biotechnology (AVT.CSB), RWTH Aachen University, Aachen, Germany., von Lieres E; Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Jülich, Germany.; Computational Systems Biotechnology (AVT.CSB), RWTH Aachen University, Aachen, Germany., Wiechert W; Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Jülich, Germany.; Computational Systems Biotechnology (AVT.CSB), RWTH Aachen University, Aachen, Germany., Reich SJ; Department of Biology, Ulm University, Ulm, Germany., Riedel CU; Department of Biology, Ulm University, Ulm, Germany., Oldiges M; Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Jülich, Germany.; Institute of Biotechnology, RWTH Aachen University, Aachen, Germany.
Jazyk: angličtina
Zdroj: Biotechnology progress [Biotechnol Prog] 2024 Jul 26, pp. e3495. Date of Electronic Publication: 2024 Jul 26.
DOI: 10.1002/btpr.3495
Abstrakt: Bacteriocins are ribosomally synthesized peptides with the innate ability to kill or inhibit growth of other bacteria. In recent years, bacteriocins have received increased interest, as their antimicrobial activity enhances food safety and shelf life by combatting pathogens such as Listeria monocytogenes. They also have application potential as an active pharmaceutical compound to combat multidrug-resistant pathogens. As new bacteriocins continue to be discovered, accelerated workflows for screening, identification, and process development have been developed. However, antimicrobial activity measurement is often still limited with regards to quantification and throughput. Here, we present the use of a non-linear calibration model to infer nisin concentrations in cultivation supernatants of Lactococcus lactis ssp. lactis B1629 using readouts of pHluorin2 fluorescence-based antimicrobial activity assays.
(© 2024 The Author(s). Biotechnology Progress published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers.)
Databáze: MEDLINE
Externí odkaz: