Visualizing intermediate stages of viral membrane fusion by cryo-electron tomography.

Autor: Kephart SM; Department of Medicinal Chemistry, University of Washington, Seattle, WA, USA., Hom N; Department of Medicinal Chemistry, University of Washington, Seattle, WA, USA., Lee KK; Department of Medicinal Chemistry, University of Washington, Seattle, WA, USA; Biological Structure Physics and Design Graduate Program, University of Washington, Seattle, WA, USA. Electronic address: kklee@uw.edu.
Jazyk: angličtina
Zdroj: Trends in biochemical sciences [Trends Biochem Sci] 2024 Oct; Vol. 49 (10), pp. 916-931. Date of Electronic Publication: 2024 Jul 24.
DOI: 10.1016/j.tibs.2024.06.012
Abstrakt: Protein-mediated membrane fusion is the dynamic process where specialized protein machinery undergoes dramatic conformational changes that drive two membrane bilayers together, leading to lipid mixing and opening of a fusion pore between previously separate membrane-bound compartments. Membrane fusion is an essential stage of enveloped virus entry that results in viral genome delivery into host cells. Recent studies applying cryo-electron microscopy techniques in a time-resolved fashion provide unprecedented glimpses into the interaction of viral fusion proteins and membranes, revealing fusion intermediate states from the initiation of fusion to release of the viral genome. In combination with complementary structural, biophysical, and computation modeling approaches, these advances are shedding new light on the mechanics and dynamics of protein-mediated membrane fusion.
Competing Interests: Declaration of interests The authors declare no competing interests.
(Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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