Antibacterial mechanism of CO 2 combined with low temperature against Shewanella putrefaciens by biochemical and metabolomics analysis.

Autor: Li P; College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; National Experimental Teaching Demonstration Center for Food Science and Engineering Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquatic Product Processing and Preservation, Shanghai 201306, China; Shanghai Professional Technology Service Platform on Cold Chain Equipment Performance and Energy Saving Evaluation, Shanghai 201306, China., Mei J; College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; National Experimental Teaching Demonstration Center for Food Science and Engineering Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquatic Product Processing and Preservation, Shanghai 201306, China; Shanghai Professional Technology Service Platform on Cold Chain Equipment Performance and Energy Saving Evaluation, Shanghai 201306, China. Electronic address: jmei@shou.edu.cn., Xie J; College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; National Experimental Teaching Demonstration Center for Food Science and Engineering Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquatic Product Processing and Preservation, Shanghai 201306, China; Shanghai Professional Technology Service Platform on Cold Chain Equipment Performance and Energy Saving Evaluation, Shanghai 201306, China; Key Laboratory of Aquatic Products High-quality Utilization, Storage and Transportation (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shanghai 201306, China. Electronic address: jxie@shou.edu.cn.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2024 Dec 01; Vol. 460 (Pt 1), pp. 140555. Date of Electronic Publication: 2024 Jul 19.
DOI: 10.1016/j.foodchem.2024.140555
Abstrakt: To further reveal the inhibition mechanism of carbon dioxide (CO 2 ) on Shewanella putrefaciens (S. putrefaciens), influence on metabolic function was studied by biochemical and metabolomics analysis. Accordingly, reduction of intracellular pH (pH i ), depolarization of cell membrane and accumulation of reactive oxygen species (ROS) indicated that CO 2 changed the membrane permeability of S. putrefaciens. Besides, adenosine triphosphate (ATP), ATPase, nicotinamide adenine dinucleotide (NAD + /NADH) and ratios of NADH/NAD + were detected, indicating a role of CO 2 in repressing respiratory pathway and electron transport. According to metabolomics results, CO 2 induced differential expressions of metabolites, disordered respiratory chain and weakened energy metabolism of S. putrefaciens. Inhibition of respiratory rate-limiting enzymes also revealed that electron transfer of respiratory chain was blocked, cell respiration was weakened, and thus energy supply was insufficient under CO 2 stress. These results revealed that CO 2 caused disruption of metabolic function, which might be the main cause of growth inhibition for S. putrefaciens.
Competing Interests: Declaration of competing interest None.
(Copyright © 2024. Published by Elsevier Ltd.)
Databáze: MEDLINE
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