Nucleotide depletion in hypoxia experimental models of mouse myocardial slices.
Autor: | Zabielska-Kaczorowska MA; Department of Physiology, Medical University of Gdansk, Gdansk, Poland., Stawarska K; Department of Biochemistry, Medical University of Gdansk, Gdansk, Poland., Kawecka A; Department of Biochemistry, Medical University of Gdansk, Gdansk, Poland., Urbanowicz K; Department of Biochemistry, Medical University of Gdansk, Gdansk, Poland., Smolenski RT; Department of Biochemistry, Medical University of Gdansk, Gdansk, Poland., Kutryb-Zajac B; Department of Biochemistry, Medical University of Gdansk, Gdansk, Poland. |
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Jazyk: | angličtina |
Zdroj: | Nucleosides, nucleotides & nucleic acids [Nucleosides Nucleotides Nucleic Acids] 2024 Jul 24, pp. 1-13. Date of Electronic Publication: 2024 Jul 24. |
DOI: | 10.1080/15257770.2024.2381791 |
Abstrakt: | Objectives: Experimental models to test the effective protection against cardiac ischemia injury are still challenging in pre-clinical studies. The use of myocardial slices creates a special link between testing isolated cardiomyocytes and whole-heart research. In this work, we investigated the effects of oxygen deprivation in a hypoxic chamber and treatment with cobalt chloride (CoCl Methods: 200 μm-thick left ventricle myocardial slices were obtained from 3-month-old male C57Bl/6J mice using an oscillatory microtome. Slices were then exposed to 1% O Key Findings: A significant effect of hypoxia with 1% O Conclusions: A short-term culture of myocardial slices in a hypoxic chamber seems to be an appropriate model of cardiac ischemia for testing new pharmacological approaches based on modulating the energy metabolism of cardiac cells. |
Databáze: | MEDLINE |
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