Novel lytic bacteriophage AhFM11 as an effective therapy against hypervirulent Aeromonas hydrophila.

Autor: Muliya Sankappa N; Department of Aquatic Animal Health Management, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, Mangaluru, Karnataka, 575002, India.; ARS Research Participation Program, Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN, 37830, USA.; Aquatic Animal Health Research Unit, United States Department of Agriculture, Agricultural Research Service, Auburn, AL, 36832, USA., Shivani Kallappa G; Department of Aquatic Animal Health Management, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, Mangaluru, Karnataka, 575002, India. skgirisha@gmail.com., Kallihosuru Boregowda K; Department of Aquatic Animal Health Management, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, Mangaluru, Karnataka, 575002, India., Mandrira Ramakrishna N; Department of Aquatic Animal Health Management, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, Mangaluru, Karnataka, 575002, India., Kattapuni Suresh P; Division of Infectious Diseases, Nitte University Centre for Science Education and Research, Mangaluru, India., Shriraje Balakrishna D; Department of Aquatic Animal Health Management, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, Mangaluru, Karnataka, 575002, India., Ballamoole KK; Division of Infectious Diseases, Nitte University Centre for Science Education and Research, Mangaluru, India., Thangavel S; Department of Aquatic Animal Health Management, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, Mangaluru, Karnataka, 575002, India., Sahoo L; Department of Fish Genetics and Reproduction, College of Fisheries, Central Agricultural University (Imphal), Lembucherra, Tripura West, Tripura, 799210, India., Lange MD; Aquatic Animal Health Research Unit, United States Department of Agriculture, Agricultural Research Service, Auburn, AL, 36832, USA., Deshotel MB; United States Department of Agriculture, Agricultural Research Service, Harry K. Dupree Stuttgart National Aquaculture Research Center, Stuttgart, AR, 72160, USA., Abernathy JW; Aquatic Animal Health Research Unit, United States Department of Agriculture, Agricultural Research Service, Auburn, AL, 36832, USA. jason.abernathy@usda.gov.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Jul 23; Vol. 14 (1), pp. 16882. Date of Electronic Publication: 2024 Jul 23.
DOI: 10.1038/s41598-024-67768-2
Abstrakt: Several farmed fish species, including carps, tilapia, salmon, and catfish, have experienced significant economic losses in aquaculture due to motile Aeromonas septicemia caused by Aeromonas hydrophila. In the present study, a novel lytic bacteriophage infecting hypervirulent Aeromonas hydrophila (vAh) was isolated and characterized. This is the first report of a phage against vAh. Phage AhFM11 demonstrated lytic activity against both vAh strains and the A. hydrophila reference strain ATCC 35654. The AhFM11 genome was sequenced and assembled, comprising 168,243 bp with an average G + C content of 41.5%. The genome did not harbor any antibiotic resistance genes. Genomic information along with transmission electron microscopy revealed that phage AhFM11 belongs to the Straboviridae family. Therapeutic application of monophage AhFM11 in fish showed 100% survival in injection, 95% in immersion and 93% in oral feeding of phage top-coated feed. Fish and chicken meat spiked with A. hydrophila and phage showed significant reduction of A. hydrophila. These findings support that phage AhFM11 can be used as a biocontrol agent against vAh as an alternative to antibiotics.
(© 2024. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje