Molecular detection and characterization of Anaplasma marginale and Babesia canis vogeli infecting dogs in Luxor, Egypt.

Autor: Mahmoud HYAH; Division of Infectious Diseases, Animal Medicine Department, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt. mhassan@vet.svu.edu.eg., Shahat MS; Division of Internal Medicine, Animal Medicine Department, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt., Fereig RM; Division of Internal Medicine, Animal Medicine Department, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt., Ali AO; Division of Infectious Diseases, Animal Medicine Department, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt., Emeish WFA; Department of Fish Diseases and Management, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt., Soliman AM; Biotechnology Department, Animal Health Research Institute, Agricultural Research Center, Dokki, 12618, Giza, Egypt.; Laboratory of Infectious Diseases, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, 890-0065, Japan., Khalifa FA; Division of Infectious Diseases, Animal Medicine Department, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt., Tanaka T; Laboratory of Infectious Diseases, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, 890-0065, Japan.; Laboratory of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University, Sendai, 980-8572, Japan.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Jul 23; Vol. 14 (1), pp. 16888. Date of Electronic Publication: 2024 Jul 23.
DOI: 10.1038/s41598-024-67009-6
Abstrakt: Tick-borne diseases in animals are increasing rapidly worldwide, but there is insufficient information about tick-borne diseases infecting dogs in southern Egypt. Thus, in the current study, we detected the presence of Anaplasma marginale (A. marginale) and Babesia canis vogeli (B. canis vogeli) in the blood of dogs. The results revealed that 4/100 (4%) were positive, and a higher infection rate was found in males (75%), than females (25%). The phylogenetic analysis for the major surface protein 4 (msp4) gene in this study was compared with amplicons separate from other reported isolates with alignment by identity 100% with cattle and camels from Egypt, and the phylogenetic analysis for the B. canis vogeli small subunit ribosomal RNA (SSU rRNA) gene in this study identified identity by 99.89% with dogs from Egypt. This report is considered the first report in southern Egypt about A. marginale in dogs based on the sequence analysis of the msp4 gene, providing new data for the classification and identification of A. marginale in dogs compared to A. marginale isolated from other animals in southern Egypt.
(© 2024. The Author(s).)
Databáze: MEDLINE
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