Blue Light Compromises Bacterial β-Lactamases Activity to Overcome β-Lactam Resistance.

Autor: Dos Anjos C; Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA., Wang Y; Division of Infectious Diseases and Medical Services, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA., Truong-Bolduc QC; Division of Infectious Diseases and Medical Services, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA., Bolduc PK; College of Engineering, University of Massachusetts, Amherst, Massachusetts, USA., Liu M; Carnegie Mellon University, Pittsburgh, Pennsylvania, USA., Hooper DC; Division of Infectious Diseases and Medical Services, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA., Anderson RR; Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA., Dai T; Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA., Leanse LG; Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.; Europa Point Campus, University of Gibraltar, Gibraltar, Gibraltar.
Jazyk: angličtina
Zdroj: Lasers in surgery and medicine [Lasers Surg Med] 2024 Sep; Vol. 56 (7), pp. 673-681. Date of Electronic Publication: 2024 Jul 22.
DOI: 10.1002/lsm.23819
Abstrakt: Objective: In this study, we evaluated the effectiveness of antimicrobial blue light (aBL; 410 nm wavelength) against β-lactamase-carrying bacteria and the effect of aBL on the activity of β-lactamases.
Methods: Pseudomonas aeruginosa, Escherichia coli, and Klebsiella pneumoniae strains carrying β-lactamases as well as a purified β-lactamase enzymes were studied. β-lactamase activity was assessed using a chromogenic cephalosporin hydrolysis assay. Additionally, we evaluated the role of porphyrins in the photoreaction, as well as protein degradation by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Finally, we investigated the bactericidal effect of combined aBL-ceftazidime exposure against a metallo-β-lactamase expressing P. aeruginosa strain.
Results: Our study demonstrated that aBL effectively killed β-lactamase-producing bacteria and reduced β-lactamase activity. After an aBL exposure of 1.52 J/cm 2 , a 50% reduction in enzymatic activity was observed in P. aeruginosa. Additionally, we found a 40% decrease in the photoreaction activity of porphyrins following an aBL exposure of 64.8 J/cm 2 . We also revealed that aBL reduced β-lactamase activity via protein degradation (after 136.4 J/cm 2 ). Additionally, aBL markedly improved the bactericidal effect of ceftazidime (by >4-log 10 ) in the metallo-β-lactamase P. aeruginosa strain.
Conclusion: Our results provide evidence that aBL compromises bacterial β-lactamase activity, offering a potential approach to overcome β-lactam resistance in bacteria.
(© 2024 Wiley Periodicals LLC.)
Databáze: MEDLINE
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