Antiviral effect of alkaloids-free Ephedra Herb extract on respiratory syncytial virus infection.

Autor:	Fujikane A; Department of General Medicine, Faculty of Medicine, Fukuoka University, Fukuoka, Japan., Fujikane R; Department of Physiological Science and Molecular Biology, Fukuoka Dental College, Fukuoka, Japan.; Oral Medicine Research Center, Fukuoka Dental College, Fukuoka, Japan., Hyuga S; Oriental Medicine Research Center, School of Pharmacy, Kitasato University, Tokyo, Japan., Sechi Y; Department of General Medicine, Faculty of Medicine, Fukuoka University, Fukuoka, Japan., Hiyoshi T; Department of General Medicine, Faculty of Medicine, Fukuoka University, Fukuoka, Japan., Sakamoto A; Department of General Medicine, Faculty of Medicine, Fukuoka University, Fukuoka, Japan., Nishi A; TSUMURA Advanced Technology Research Laboratories, Tsumura & Co., Ibaraki, Japan., Odaguchi H; Oriental Medicine Research Center, School of Pharmacy, Kitasato University, Tokyo, Japan., Hiromatsu K; Department of Microbiology and Immunology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan., Goda Y; National Institute of Health Sciences, Kawasaki, Kanagawa, Japan., Ishino Y; Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan., Nabeshima S; Department of General Medicine, Faculty of Medicine, Fukuoka University, Fukuoka, Japan.
Jazyk:	angličtina
Zdroj:	Frontiers in pharmacology [Front Pharmacol] 2024 Jul 05; Vol. 15, pp. 1410470. Date of Electronic Publication: 2024 Jul 05 (Print Publication: 2024).
DOI:	10.3389/fphar.2024.1410470
Abstrakt:	Respiratory syncytial virus (RSV) is a major cause of respiratory tract infection in children. Despite decades of efforts, no effective therapies are available. We recently reported that extracts of Ephedra Herb and Cinnamon Bark interacted with the G attachment protein of RSV to inhibit infectivity. The present in vitro study aimed to investigate the antiviral effect of ephedrine alkaloids-free Ephedra Herb extract (EFE), which is characterized by free of harmful effects of ephedrine alkaloids in Ephedra Herb, on experimental RSV infection. Infection of RSV into A549 cells simultaneously with EFE resulted the significant reduction of RSV RNA, viral protein, and viral titers after the incubation of the cells. We found that RSV attachment to the cell surface was inhibited both in the presence of EFE and when RSV particles were pre-treated with EFE. We also found that EFE specifically interacted with the central conserved domain of RSV G protein by surface plasmon resonance, demonstrating that specific binding of G protein to the cellular receptor was inhibited by EFE. Another mechanism was found in which a higher concentration of EFE inhibited the viral load immediately after the viral entry into host cells, suggesting the inhibition of viral RNA replication. These results demonstrate that EFE worked against RSV infection through multiple antiviral mechanisms, a unique feature of this crude drug extract. Competing Interests: AN was employed by Tsumura & Co. SN has financial interests in Tsumura & Co. relevant to this research. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision (Copyright © 2024 Fujikane, Fujikane, Hyuga, Sechi, Hiyoshi, Sakamoto, Nishi, Odaguchi, Hiromatsu, Goda, Ishino and Nabeshima.)
Databáze:	MEDLINE
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