Characterization of mycobacteriophage Adephagia cytotoxic proteins.

Autor: Freeman KG; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA., Lauer MJ; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA., Jiang D; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA., Roscher J; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA., Sandler S; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA., Mercado N; Department of Biological Sciences, University of North Texas, Denton, TX 76203, USA., Fryberger R; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA., Kovalski J; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA., Lutz AR; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA., Hughes LE; Department of Biological Sciences, University of North Texas, Denton, TX 76203, USA., VanDemark AP; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA., Hatfull GF; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA.
Jazyk: angličtina
Zdroj: G3 (Bethesda, Md.) [G3 (Bethesda)] 2024 Sep 04; Vol. 14 (9).
DOI: 10.1093/g3journal/jkae166
Abstrakt: Mycobacterium phage Adephagia is a cluster K phage that infects Mycobacterium smegmatis and some strains of Mycobacterium pathogens. Adephagia has a siphoviral virion morphology and is temperate. Its genome is 59,646 bp long and codes for one tRNA gene and 94 predicted protein-coding genes; most genes not associated with virion structure and assembly are functionally ill-defined. Here, we determined the Adephagia gene expression patterns in lytic and lysogenic growth and used structural predictions to assign additional gene functions. We characterized 66 nonstructural genes for their toxic phenotypes when expressed in M. smegmatis, and we show that 25 of these (38%) are either toxic or strongly inhibit growth, resulting in either reduced viability or small colony sizes. Some of these genes are predicted to be involved in DNA metabolism or regulation, but others are of unknown function. We also characterize the HicAB-like toxin-antitoxin (TA) system encoded by Adephagia (gp91 and gp90, respectively) and show that the gp90 antitoxin is lysogenically expressed, abrogates gp91 toxicity, and is required for normal lytic and lysogenic growth.
Competing Interests: Conflicts of interest The author(s) declare no conflicts of interest.
(© The Author(s) 2024. Published by Oxford University Press on behalf of The Genetics Society of America.)
Databáze: MEDLINE
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