Baseline and stress-induced changes in plasma bacterial killing ability against gram-negative bacteria are partially mediated by the complement system in Rhinella diptycha toads.

Autor: de Oliveira Rosa SA; Department of Physiology, Institute of Bioscience, University of São Paulo, São Paulo, Brazil., Titon Junior B; Department of Physiology, Institute of Bioscience, University of São Paulo, São Paulo, Brazil., de Figueiredo AC; Department of Physiology, Institute of Bioscience, University of São Paulo, São Paulo, Brazil., Lima AS; Department of Physiology, Institute of Bioscience, University of São Paulo, São Paulo, Brazil., Gomes FR; Department of Physiology, Institute of Bioscience, University of São Paulo, São Paulo, Brazil., Titon SCM; Department of Physiology, Institute of Bioscience, University of São Paulo, São Paulo, Brazil. Electronic address: stefannychristie@gmail.com.
Jazyk: angličtina
Zdroj: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology [Comp Biochem Physiol A Mol Integr Physiol] 2024 Nov; Vol. 297, pp. 111701. Date of Electronic Publication: 2024 Jul 17.
DOI: 10.1016/j.cbpa.2024.111701
Abstrakt: The plasma bacterial killing ability (BKA) is modulated by the stress response in vertebrates, including amphibians. The complement system is an effector mechanism comprised of a set of proteins present in the plasma that once activated can promote bacterial lysis. Herein, we investigated whether changes in plasma BKA as a result of the acute stress response and an immune challenge are mediated by the complement system in Rhinella diptycha toads. Additionally, we investigated whether the observed changes in plasma BKA are associated with changes in plasma corticosterone levels (CORT). We subjected adult male toads to a restraint or an immune challenge (with three concentrations of Aeromonas hydrophila heat inactivated), and then evaluated the plasma BKA against A. hydrophila, in vitro. We determined the complement system activity on plasma BKA, by treating the plasma (baseline, 1 h and 24 h post-restraint, and after the immune challenge) with ethylenediaminetetraacetic acid, heat, or protease. Our results showed increased CORT 1 h and 24 h after restraint and decreased plasma BKA 24 h post-restraint. The inhibitors of the complement system decreased the plasma BKA compared with untreated plasma at all times (baseline, 1 h, and 24 h after restraint), demonstrating that the plasma BKA activity is partially mediated by the complement system. The immune challenge increased CORT, with the highest values being observed in the highest bacterial concentration, compared with control. The plasma BKA was not affected by the immune challenge but was demonstrated to be partially mediated by the complement system. Our results demonstrated that restraint and the immune challenge activated the hypothalamus-pituitary-interrenal axis, by increasing plasma CORT levels in R. diptycha. Also, our results demonstrated the complement system is participative in the plasma BKA for baseline and post-stress situations in these toads.
Competing Interests: Declaration of competing interest The authors have no competing interests to declare.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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