The differential formation and composition of leukocyte-platelet aggregates induced by various cellular stimulants.

Autor: Peshkova AD; Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russian Federation., Saliakhutdinova SM; Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russian Federation., Sounbuli K; Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russian Federation., Selivanova YA; Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russian Federation., Andrianova IA; Department of Internal Medicine, Division of Hematology and Program in Molecular Medicine, University of Utah, Salt Lake City, UT, USA., Khabirova AI; Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russian Federation., Litvinov RI; Departments of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA, USA., Weisel JW; Departments of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA, USA. Electronic address: weisel@pennmedicine.upenn.edu.
Jazyk: angličtina
Zdroj: Thrombosis research [Thromb Res] 2024 Sep; Vol. 241, pp. 109092. Date of Electronic Publication: 2024 Jul 07.
DOI: 10.1016/j.thromres.2024.109092
Abstrakt: Background: Leukocyte-platelet aggregates comprise a pathogenic link between hemostasis and immunity, but the prerequisites and mechanisms of their formation remain not understood.
Aims: To quantify the formation, composition, and morphology of leukocyte-platelet aggregates in vitro under the influence of various cellular activators.
Methods: Phorbol-12-myristate-13-acetate (PMA), lipopolysaccharide (LPS), thrombin receptor-activating peptide (TRAP-6), and adenosine diphosphate (ADP) were used as cellular activators. Flow cytometry was utilized to identify and quantify aggregates in whole human blood and platelet-rich plasma. Cell types and cellular aggregates were identified using fluorescently labeled antibodies against the appropriate cellular markers, and cell activation was assessed by the expression of appropriate surface markers. For confocal fluorescent microscopy, cell membranes and nuclei were labeled. Neutrophil-platelet aggregates were studied using scanning electron microscopy.
Results: In the presence of PMA, ADP or TRAP-6, about 17-38 % of neutrophils and 61-77 % of monocytes formed aggregates with platelets in whole blood, whereas LPS did not induce platelet aggregation with either neutrophils or monocytes due the inability to activate platelets. Similar results were obtained when isolated neutrophils were added to platelet-rich plasma. All the cell types involved in the heterotypic aggregation expressed molecular markers of activation. Fluorescent and electron microscopy of the aggregates showed that the predominant platelet/leukocyte ratios were 1:1 and 2:1.
Conclusions: Formation of leukocyte-platelet aggregates depends on the nature of the cellular activator and the spectrum of its cell-activating ability. An indispensable condition for formation of leukocyte-platelet aggregates is activation of all cell types including platelets, which is the restrictive step.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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