Analysis of glycoalkaloid distribution in the tissues of mealworm larvae (Tenebrio molitor).

Autor: Winkiel MJ; Department of Animal Physiology and Developmental Biology, Faculty of Biology, Institute of Experimental Biology, Adam Mickiewicz University in Poznań, Uniwersytetu Poznańskiego 6, 61-614, Poznan, Poland. magwin@amu.edu.pl., Chowański S; Department of Animal Physiology and Developmental Biology, Faculty of Biology, Institute of Experimental Biology, Adam Mickiewicz University in Poznań, Uniwersytetu Poznańskiego 6, 61-614, Poznan, Poland., Sulli M; Italian National Agency for New Technologies, Energy and Sustainable Development ENEA, Via Anguillarese 301, 00123, Roma, Italy., Diretto G; Italian National Agency for New Technologies, Energy and Sustainable Development ENEA, Via Anguillarese 301, 00123, Roma, Italy., Słocińska M; Department of Animal Physiology and Developmental Biology, Faculty of Biology, Institute of Experimental Biology, Adam Mickiewicz University in Poznań, Uniwersytetu Poznańskiego 6, 61-614, Poznan, Poland.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Jul 17; Vol. 14 (1), pp. 16540. Date of Electronic Publication: 2024 Jul 17.
DOI: 10.1038/s41598-024-67258-5
Abstrakt: Solanine (SOL) and chaconine (CHA) are glycoalkaloids (GAs) produced mainly by Solanum plants. These plant secondary metabolites affect insect metabolism; thus, they have the potential to be applied as natural plant protection products. However, it is not known which GA concentration induces physiological changes in animals. Therefore, the aim of this study was to perform a quantitative analysis of SOL and CHA in the larvae of Tenebrio molitor using LC‒MS to assess how quickly they are eliminated or metabolised. In this experiment, the beetles were injected with 2 μL of 10 -5  M SOL or CHA solution, which corresponds to a dosage range of 0.12-0.14 ng/mg body mass. Then, 0.5, 1.5, 8, and 24 h after GA application, the haemolymph (H), gut (G), and the remainder of the larval body (FB) were isolated. GAs were detected in all samples tested for 24 h, with the highest percentage of the amount applied in the FB, while the highest concentration was measured in the H sample. The SOL and CHA concentrations decreased in the haemolymph over time, while they did not change in other tissues. CHA had the highest elimination rate immediately after injection, while SOL slightly later. None of the GA hydrolysis products were detected in the tested samples. One possible mechanism of the detoxification of GAs may be oxidation and/or sequestration. They may be excreted by Malpighian tubules, with faeces or with cuticles during moulting. The results presented are significant because they facilitate the interpretation of studies related to the effects of toxic substances on insect metabolism.
(© 2024. The Author(s).)
Databáze: MEDLINE
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