Inflammatory responses in Atlantic lumpfish (Cyclopterus lumpus L.) after intraperitoneal injection of a vaccine against Aeromonas salmonicida and Vibrio salmonicida at different water temperatures.

Autor: Knutsen IS; Faculty of Biosciences, Fisheries and Economics, Norwegian College of Fishery Science, UiT the Arctic University of Norway, Tromsø, Norway., Erkinharju T; Faculty of Biosciences, Fisheries and Economics, Norwegian College of Fishery Science, UiT the Arctic University of Norway, Tromsø, Norway., Bøgwald J; Faculty of Biosciences, Fisheries and Economics, Norwegian College of Fishery Science, UiT the Arctic University of Norway, Tromsø, Norway., Dalmo RA; Faculty of Biosciences, Fisheries and Economics, Norwegian College of Fishery Science, UiT the Arctic University of Norway, Tromsø, Norway., Seternes T; Faculty of Biosciences, Fisheries and Economics, Norwegian College of Fishery Science, UiT the Arctic University of Norway, Tromsø, Norway.
Jazyk: angličtina
Zdroj: Journal of fish diseases [J Fish Dis] 2024 Nov; Vol. 47 (11), pp. e14001. Date of Electronic Publication: 2024 Jul 16.
DOI: 10.1111/jfd.14001
Abstrakt: Studying inflammatory responses induced by vaccination can contribute to a more detailed understanding of underlying immune mechanisms in lumpfish (Cyclopterus lumpus). Tissue samples from lumpfish intraperitoneally immunized with a divalent oil-adjuvanted vaccine (Aeromonas salmonicida and Vibrio salmonicida) at water temperatures of 5, 10, and 15°C were collected at 630 day degrees and 18 weeks post injection. The relative amount of secretory and membrane-bound immunoglobulin M (IgM) gene transcripts in the head kidney was determined by qPCR. Vaccine-induced inflammatory lesions were assessed on histological sections of abdominal pancreatic/intestinal tissue from vaccinated fish in all three temperature groups. Inflammatory cells forming dense aggregations in lesions showed proliferative activity, many of which were identified as eosinophilic-granulocyte-like cells. IgM+ cells were scattered in inflammatory tissue dominated by connective tissue, showing no difference in numbers between lesions from fish vaccinated at 5, 10, and 15°C. Relative gene expression analysis of secretory and membrane-bound IgM revealed low overall expression in the head kidney of vaccinated fish at both 630 day-degrees and 18 weeks post injection. The results of this study indicate that the vaccine stimulated prolonged local inflammatory responses at the injection site, which were not influenced by temperature.
(© 2024 The Author(s). Journal of Fish Diseases published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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