Cytokinetic abscission in Toxoplasma gondii is governed by protein phosphatase 2A and the daughter cell scaffold complex.

Autor:	Marq JB; Department of Microbiology and Molecular Medicine, University of Geneva, Geneva, Switzerland., Gosetto M; Department of Microbiology and Molecular Medicine, University of Geneva, Geneva, Switzerland., Altenried A; Department of Microbiology and Molecular Medicine, University of Geneva, Geneva, Switzerland., Vadas O; Department of Microbiology and Molecular Medicine, University of Geneva, Geneva, Switzerland., Maco B; Department of Microbiology and Molecular Medicine, University of Geneva, Geneva, Switzerland., Dos Santos Pacheco N; Department of Microbiology and Molecular Medicine, University of Geneva, Geneva, Switzerland., Tosetti N; Department of Microbiology and Molecular Medicine, University of Geneva, Geneva, Switzerland., Soldati-Favre D; Department of Microbiology and Molecular Medicine, University of Geneva, Geneva, Switzerland. dominique.soldati-favre@unige.ch., Lentini G; Department of Microbiology and Molecular Medicine, University of Geneva, Geneva, Switzerland. gaelle.lentini@unibe.ch.; Institute of Cell Biology, University of Bern, Bern, Switzerland. gaelle.lentini@unibe.ch.
Jazyk:	angličtina
Zdroj:	The EMBO journal [EMBO J] 2024 Sep; Vol. 43 (17), pp. 3752-3786. Date of Electronic Publication: 2024 Jul 15.
DOI:	10.1038/s44318-024-00171-9
Abstrakt:	Cytokinetic abscission marks the final stage of cell division, during which the daughter cells physically separate through the generation of new barriers, such as the plasma membrane or cell wall. While the contractile ring plays a central role during cytokinesis in bacteria, fungi and animal cells, the process diverges in Apicomplexa. In Toxoplasma gondii, two daughter cells are formed within the mother cell by endodyogeny. The mechanism by which the progeny cells acquire their plasma membrane during the disassembly of the mother cell, allowing daughter cells to emerge, remains unknown. Here we identify and characterize five T. gondii proteins, including three protein phosphatase 2A subunits, which exhibit a distinct and dynamic localization pattern during parasite division. Individual downregulation of these proteins prevents the accumulation of plasma membrane at the division plane, preventing the completion of cellular abscission. Remarkably, the absence of cytokinetic abscission does not hinder the completion of subsequent division cycles. The resulting progeny are able to egress from the infected cells but fail to glide and invade, except in cases of conjoined twin parasites. (© 2024. The Author(s).)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu