High-throughput label-free opioid receptor binding assays using an automated desorption electrospray ionization mass spectrometry platform.

Autor: Feng Y; Department of Chemistry, Bindley Bioscience Center, and Purdue Institute for Cancer Research, Purdue University, West Lafayette, IN 47907, USA. cooks@purdue.edu., Morato NM; Department of Chemistry, Bindley Bioscience Center, and Purdue Institute for Cancer Research, Purdue University, West Lafayette, IN 47907, USA. cooks@purdue.edu., Huang KH; Department of Chemistry, Bindley Bioscience Center, and Purdue Institute for Cancer Research, Purdue University, West Lafayette, IN 47907, USA. cooks@purdue.edu., Lin M; Department of Chemistry, Bindley Bioscience Center, and Purdue Institute for Cancer Research, Purdue University, West Lafayette, IN 47907, USA. cooks@purdue.edu., Cooks RG; Department of Chemistry, Bindley Bioscience Center, and Purdue Institute for Cancer Research, Purdue University, West Lafayette, IN 47907, USA. cooks@purdue.edu.
Jazyk: angličtina
Zdroj: Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2024 Aug 01; Vol. 60 (63), pp. 8224-8227. Date of Electronic Publication: 2024 Aug 01.
DOI: 10.1039/d4cc02346c
Abstrakt: The current opioid epidemic has incentivized the discovery of new non-addictive analgesics, a process that requires the screening of opioid receptor binding, traditionally performed using radiometric assays. Here we describe a label-free alternative based on high-throughput (1 Hz) ambient mass spectrometry for screening the receptor binding of new opioid analogues.
Databáze: MEDLINE