Newly developed peptide-ELISA successfully detected anti-IgG antibodies against Maedi-Visna virus in sheep.

Autor: Koçkaya ES; Ege University Faculty of Science Department of Biology Molecular Biology Section, İzmir, Türkiye., Can H; Ege University Faculty of Science Department of Biology Molecular Biology Section, İzmir, Türkiye; Ege University Vaccine Development Application and Research Center, İzmir, Türkiye. Electronic address: huseyin.can@ege.edu.tr., Yaman Y; Siirt University Faculty of Veterinary Medicine, Department of Genetics, Siirt, Türkiye., Kandemir Ç; Ege University Faculty of Agriculture, Department of Animal Science, İzmir, Türkiye., Taşkın T; Ege University Faculty of Agriculture, Department of Animal Science, İzmir, Türkiye., Karakavuk M; Ege University Vaccine Development Application and Research Center, İzmir, Türkiye; Ege University Ödemiş Vocational School, İzmir, Türkiye., Değirmenci Döşkaya A; Ege University Vaccine Development Application and Research Center, İzmir, Türkiye; Ege University Faculty of Medicine, Department of Parasitology, İzmir, Türkiye., Döşkaya M; Ege University Vaccine Development Application and Research Center, İzmir, Türkiye; Ege University Faculty of Medicine, Department of Parasitology, İzmir, Türkiye., Pehlivan E; Ankara University Faculty of Agriculture, Department of Animal Science, Ankara, Türkiye., Şireli HD; Dicle University Faculty of Agriculture, Department of Animal Science, Diyarbakır, Türkiye., Gürüz AY; Ege University Vaccine Development Application and Research Center, İzmir, Türkiye; Ege University Faculty of Medicine, Department of Parasitology, İzmir, Türkiye., Ün C; Ege University Faculty of Science Department of Biology Molecular Biology Section, İzmir, Türkiye; Ege University Vaccine Development Application and Research Center, İzmir, Türkiye.
Jazyk: angličtina
Zdroj: Veterinary immunology and immunopathology [Vet Immunol Immunopathol] 2024 Aug; Vol. 274, pp. 110806. Date of Electronic Publication: 2024 Jul 10.
DOI: 10.1016/j.vetimm.2024.110806
Abstrakt: Maedi Visna Virus (MVV) is a retrovirus that can infect sheep. There is still no effective therapy or vaccine against this virus and timely diagnosis is important to combat the complications of the disease. In this study, we aimed to develop an ELISA using peptides derived from gag protein as antigen. For this purpose, B cell epitopes of gag protein were predicted and a docking analysis with the B cell receptor was performed to select peptides to be used in ELISA. After three soluble epitopes with the highest antigenicity were produced as peptides, the immunogenicity of each peptide was determined by ELISA using sheep serum samples categorized as MVV positive (n=24) and negative (n=13). Subsequently, in house ELISA using above mentioned immunogenic peptides as antigen was used to investigate MVV seroprevalence in sheep (n=88). According to the results, among three peptides, two of them strongly reacted with MVV positive serum samples and the mean absorbance values detected among positive and negative serum samples were statistically significant, indicating that these peptides were immunogenic (P=0.016 and P=0.038). The third peptide also reacted with positive serum samples but the mean absorbance value was not statistically significant and this peptide was considered non-immunogenic (P=0.175). The immunogenic two peptides showed the same high sensitivity and specificity values of 91.60 and 92.80 according to the commercial kit. Moreover, MVV seroprevalence detected by peptide-ELISAs using CKQGSKE and CRPQGKAGHKG peptides as antigen was 3.40 % and 4.5 %, respectively. As a result, it was shown that these peptides can be successfully used for serological diagnosis of MVV.
Competing Interests: Declaration of Competing Interest The authors declare no conflict of interest
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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