| Autor: |
Zgórecka W; Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznan, Poland., Kranc W; Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznan, Poland., Blatkiewicz M; Department of Histology and Embryology, Poznan University of Medical Sciences, 60-812 Poznan, Poland., Kamiński K; Department of Histology and Embryology, Poznan University of Medical Sciences, 60-812 Poznan, Poland., Farzaneh M; Fertility, Infertility and Perinatology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran., Bryja A; Division of Anatomy, Department of Human Morphology and Embryology, Faculty of Medicine, Wroclaw Medical University, 50-368 Wroclaw, Poland., Mozdziak P; Physiology Graduate Program, North Carolina State University, Raleigh, NC 27695, USA.; Prestage Department of Poultry Science, North Carolina State University, Raleigh, NC 27695, USA., Antosik P; Department of Veterinary Surgery, Nicolaus Copernicus University in Toruń, 87-100 Toruń, Poland., Zabel M; Division of Anatomy and Histology, University of Zielona Góra, 65-417 Zielona Góra, Poland.; Division of Histology and Embryology, Department of Human Morphology and Embryology Faculty of Medicine, Wroclaw Medical University, 50-368 Wroclaw, Poland., Podhorska-Okołów M; Division of Ultrastructural Research, Faculty of Medicine, Wroclaw Medical University, 50-368 Wroclaw, Poland., Dzięgiel P; Division of Histology and Embryology, Department of Human Morphology and Embryology Faculty of Medicine, Wroclaw Medical University, 50-368 Wroclaw, Poland., Kempisty B; Division of Anatomy, Department of Human Morphology and Embryology, Faculty of Medicine, Wroclaw Medical University, 50-368 Wroclaw, Poland.; Physiology Graduate Program, North Carolina State University, Raleigh, NC 27695, USA.; Department of Veterinary Surgery, Nicolaus Copernicus University in Toruń, 87-100 Toruń, Poland.; Center of Assisted Reproduction, Department of Obstetrics and Gynecology, University Hospital and Masaryk University, 602 00 Brno, Czech Republic., Bukowska D; Department of Diagnostics and Clinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in Toruń, 87-100 Toruń, Poland. |
| Abstrakt: |
The oviduct provides an optimal environment for the final preparation, transport, and survival of gametes, the fertilization process, and early embryonic development. Most of the studies on reproduction are based on in vitro cell culture models because of the cell's accessibility. It creates opportunities to explore the complexity of directly linked processes between cells. Previous studies showed a significant expression of genes responsible for cell differentiation, maturation, and development during long-term porcine oviduct epithelial cells (POECs) in vitro culture. This study aimed at establishing the transcriptomic profile and comprehensive characteristics of porcine oviduct epithelial cell in vitro cultures, to compare changes in gene expression over time and deliver information about the expression pattern of genes highlighted in specific GO groups. The oviduct cells were collected after 7, 15, and 30 days of in vitro cultivation. The transcriptomic profile of gene expression was compared to the control group (cells collected after the first day). The expression of COL1A2 and LOX was enhanced, while FGFBP1 , SERPINB2 , and OVGP1 were downregulated at all selected intervals of cell culture in comparison to the 24-h control ( p -value < 0.05). Adding new detailed information to the reproductive biology field about the diversified transcriptome profile in POECs may create new future possibilities in infertility treatments, including assisted reproductive technique (ART) programmes, and may be a valuable tool to investigate the potential role of oviduct cells in post-ovulation events. |
