| Autor: |
Lo Giudice A; Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126 Perugia, Italy., Porcellato I; Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126 Perugia, Italy., Pellegrini M; Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche 'Togo Rosati', Via G. Salvemini 1, 06126 Perugia, Italy., Rottenberg S; Institute of Animal Pathology, Vetsuisse Faculty, University of Bern, Länggassstrasse 120, 3012 Bern, Switzerland., He C; Institute of Animal Pathology, Vetsuisse Faculty, University of Bern, Länggassstrasse 120, 3012 Bern, Switzerland., Dentini A; Clinica Veterinaria Tyrus, Strada delle Campore 30L, 05100 Terni, Italy., Moretti G; Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126 Perugia, Italy., Cagiola M; Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche 'Togo Rosati', Via G. Salvemini 1, 06126 Perugia, Italy., Mechelli L; Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126 Perugia, Italy., Chiaradia E; Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126 Perugia, Italy., Brachelente C; Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126 Perugia, Italy. |
| Abstrakt: |
Oral melanomas are the most common oral malignancies in dogs and are characterized by an aggressive nature, invasiveness, and poor prognosis. With biological and genetic similarities to human oral melanomas, they serve as a valuable spontaneous comparative model. Primary cell cultures are widely used in human medicine and, more recently, in veterinary medicine to study tumorigenesis, cancer progression, and innovative therapeutic approaches. This study aims to establish two- and three-dimensional primary cell lines from oral canine melanomas using fine-needle aspiration as a minimally invasive sampling method. For this study, samples were collected from six dogs, represented by four primary oral melanomas and five lymph nodal metastases. The cells were digested to obtain single-cell suspensions, seeded in flasks, or processed with Matrigel ® to form organoids. The cell cultures were characterized through flow cytometry using antibodies against Melan-A, PNL2, and Sox-10. This technique offers a minimally invasive means to obtain cell samples, particularly beneficial for patients that are ineligible for surgical procedures, and enables the establishment of in vitro models crucial for comparative studies in mucosal melanoma oncology. To the best of our knowledge, this is the first work establishing neoplastic primary cell cultures via fine-needle aspiration in dogs. |
