Germline β-1,3-glucan deposits are required for female gametogenesis in Arabidopsis thaliana.

Autor: Pinto SC; LAQV REQUIMTE, Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, rua do Campo Alegre s/n, 4169-007, Porto, Portugal.; Waite Research Institute, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, SA, 5064, Australia.; Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK., Leong WH; Waite Research Institute, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, SA, 5064, Australia.; Australian Research Council Centre of Excellence in Plant Cell Walls, University of Adelaide, Urrbrae, SA, 5064, Australia., Tan H; Waite Research Institute, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, SA, 5064, Australia.; Australian Research Council Centre of Excellence in Plant Cell Walls, University of Adelaide, Urrbrae, SA, 5064, Australia., McKee L; Department of Chemistry, Division of Glycoscience, KTH Royal Institute of Technology, Stockholm, Sweden., Prevost A; Australian Research Council Centre of Excellence in Plant Cell Walls, University of Adelaide, Urrbrae, SA, 5064, Australia., Ma C; Waite Research Institute, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, SA, 5064, Australia., Shirley NJ; Waite Research Institute, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, SA, 5064, Australia.; Australian Research Council Centre of Excellence in Plant Cell Walls, University of Adelaide, Urrbrae, SA, 5064, Australia., Petrella R; Dipartimento di Bioscienze, Università Degli Studi di Milano, Via Celoria 26, 20133, Milan, Italy., Yang X; Waite Research Institute, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, SA, 5064, Australia., Koltunow AM; Centre for Crop Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, QLD, 4072, Australia., Bulone V; Waite Research Institute, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, SA, 5064, Australia.; Australian Research Council Centre of Excellence in Plant Cell Walls, University of Adelaide, Urrbrae, SA, 5064, Australia.; Department of Chemistry, Division of Glycoscience, KTH Royal Institute of Technology, Stockholm, Sweden.; College of Medicine and Public Health, Flinders University, Bedford Park Campus, Sturt Road, Bedford Park, SA, 5042, Australia., Kanaoka MM; Institute of Transformative Bio-Molecules, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8601, Japan.; Faculty of Bioresource Sciences, Prefectural University of Hiroshima, 5562 Nanatsuka-cho, Shobara City, Hiroshima, 727-0023, Japan., Higashyiama T; Institute of Transformative Bio-Molecules, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8601, Japan.; Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan., Coimbra S; LAQV REQUIMTE, Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, rua do Campo Alegre s/n, 4169-007, Porto, Portugal., Tucker MR; Waite Research Institute, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, SA, 5064, Australia. matthew.tucker@adelaide.edu.au.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Jul 12; Vol. 15 (1), pp. 5875. Date of Electronic Publication: 2024 Jul 12.
DOI: 10.1038/s41467-024-50143-0
Abstrakt: Correct regulation of intercellular communication is a fundamental requirement for cell differentiation. In Arabidopsis thaliana, the female germline differentiates from a single somatic ovule cell that becomes encased in β-1,3-glucan, a water insoluble polysaccharide implicated in limiting pathogen invasion, regulating intercellular trafficking in roots, and promoting pollen development. Whether β-1,3-glucan facilitates germline isolation and development has remained contentious, since limited evidence is available to support a functional role. Here, transcriptional profiling of adjoining germline and somatic cells revealed differences in gene expression related to β-1,3-glucan metabolism and signalling through intercellular channels (plasmodesmata). Dominant expression of a β-1,3-glucanase in the female germline transiently perturbed β-1,3-glucan deposits, allowed intercellular movement of tracer molecules, and led to changes in germline gene expression and histone marks, eventually leading to termination of germline development. Our findings indicate that germline β-1,3-glucan fulfils a functional role in the ovule by insulating the primary germline cell, and thereby determines the success of downstream female gametogenesis.
(© 2024. The Author(s).)
Databáze: MEDLINE
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