Chondroitin sulfate glycan sulfation patterns influence histochemical labeling of perineuronal nets: a comparative study of interregional distribution in human and mouse brain.

Autor:	Belliveau C; McGill Group for Suicide Studies, Douglas Mental Health University Institute, McGill University, 6875 Blvd LaSalle, H4H 1R3, Montreal, QC, Canada.; Integrated Program in Neuroscience, McGill University, 1033 Av des Pins Ouest, H3A 1A1, Montreal, QC, Canada., Théberge S; McGill Group for Suicide Studies, Douglas Mental Health University Institute, McGill University, 6875 Blvd LaSalle, H4H 1R3, Montreal, QC, Canada.; Integrated Program in Neuroscience, McGill University, 1033 Av des Pins Ouest, H3A 1A1, Montreal, QC, Canada., Netto S; McGill Group for Suicide Studies, Douglas Mental Health University Institute, McGill University, 6875 Blvd LaSalle, H4H 1R3, Montreal, QC, Canada., Rahimian R; McGill Group for Suicide Studies, Douglas Mental Health University Institute, McGill University, 6875 Blvd LaSalle, H4H 1R3, Montreal, QC, Canada., Fakhfouri G; Department of Psychiatry, McGill University, 1033 Av des Pins Ouest, H3A 1A1, Montreal, QC, Canada., Hosdey C; McGill Group for Suicide Studies, Douglas Mental Health University Institute, McGill University, 6875 Blvd LaSalle, H4H 1R3, Montreal, QC, Canada.; Integrated Program in Neuroscience, McGill University, 1033 Av des Pins Ouest, H3A 1A1, Montreal, QC, Canada., Davoli MA; McGill Group for Suicide Studies, Douglas Mental Health University Institute, McGill University, 6875 Blvd LaSalle, H4H 1R3, Montreal, QC, Canada., Hendrickson A; Department of Medicinal Chemistry, University of Washington, 1959 NE Pacific Street, Box 357610, Seattle, WA 98195, United States., Hao K; Health and Human Sciences, University of Southern California, Zonal Avenue, Biggy St, Los Angeles, CA 90033, United States., Giros B; Department of Psychiatry, McGill University, 1033 Av des Pins Ouest, H3A 1A1, Montreal, QC, Canada., Turecki G; McGill Group for Suicide Studies, Douglas Mental Health University Institute, McGill University, 6875 Blvd LaSalle, H4H 1R3, Montreal, QC, Canada.; Integrated Program in Neuroscience, McGill University, 1033 Av des Pins Ouest, H3A 1A1, Montreal, QC, Canada.; Department of Psychiatry, McGill University, 1033 Av des Pins Ouest, H3A 1A1, Montreal, QC, Canada., Alonge KM; Department of Medicinal Chemistry, University of Washington, 1959 NE Pacific Street, Box 357610, Seattle, WA 98195, United States., Mechawar N; McGill Group for Suicide Studies, Douglas Mental Health University Institute, McGill University, 6875 Blvd LaSalle, H4H 1R3, Montreal, QC, Canada.; Integrated Program in Neuroscience, McGill University, 1033 Av des Pins Ouest, H3A 1A1, Montreal, QC, Canada.; Department of Psychiatry, McGill University, 1033 Av des Pins Ouest, H3A 1A1, Montreal, QC, Canada.
Jazyk:	angličtina
Zdroj:	Glycobiology [Glycobiology] 2024 Jun 22; Vol. 34 (8).
DOI:	10.1093/glycob/cwae049
Abstrakt:	Perineuronal nets (PNNs) are a condensed subtype of extracellular matrix that form a net-like coverings around certain neurons in the brain. PNNs are primarily composed of chondroitin sulfate (CS) proteoglycans from the lectican family that consist of CS-glycosaminoglycan side chains attached to a core protein. CS disaccharides can exist in various isoforms with different sulfation patterns. Literature suggests that CS disaccharide sulfation patterns can influence the function of PNNs as well as their labeling. This study was conducted to characterize such interregional CS disaccharide sulfation pattern differences in adult human (n = 81) and mouse (n = 19) brains. Liquid chromatography tandem mass spectrometry was used to quantify five different CS disaccharide sulfation patterns, which were then compared to immunolabeling of PNNs using Wisteria Floribunda Lectin (WFL) to identify CS-glycosaminoglycans and anti-aggrecan to identify CS proteoglycans. In healthy brains, significant regional and species-specific differences in CS disaccharide sulfation and single versus double-labeling pattern were identified. A secondary analysis to investigate how early-life stress impacts these PNN features discovered that although early-life stress increases WFL+ PNN density, the CS-glycosaminoglycan sulfation code and single versus double PNN-labeling distributions remained unaffected in both species. These results underscore PNN complexity in traditional research, emphasizing the need to consider their heterogeneity in future experiments. (© The Author(s) 2024. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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