Whole-Transcriptome Sequencing-Based Profiling of the Cutaneous Virome in Patients with Secondary Immunodeficiency.

Autor:	Youssefian L; Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, California, USA.; Center for Applied Genomics, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA., Saeidian AH; Center for Applied Genomics, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.; Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA., Saffarian Z; Imam Khomeini Hospital, Tehran University of Medical Science, Tehran, Iran.; Department of Dermatology, Razi Hospital, Tehran University of Medical Sciences, Tehran, Iran., Ariamanesh M; Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA., Abdollahimajd F; Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Molkara S; Department of Dermatology, Gonabad University of Medical Sciences, Gonabad, Iran., Shahidi-Dadras M; Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Diab R; Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Vahidnezhad F; UCSC Silicon Valley Extension, University of California, Santa Cruz, California, USA., Zeinali S; Kawsar Human Genetics Research Center, Tehran, Iran., Béziat V; St Giles Laboratory of Human Genetics of Infectious Diseases, The Rockefeller University, New York, New York, USA.; Laboratory of Human Genetics of Infectious Diseases, Necker Branch, Inserm U1163, Necker Hospital for Sick Children, Paris, France.; Imagine Institute, Paris University, Paris, France., Jouanguy E; St Giles Laboratory of Human Genetics of Infectious Diseases, The Rockefeller University, New York, New York, USA.; Laboratory of Human Genetics of Infectious Diseases, Necker Branch, Inserm U1163, Necker Hospital for Sick Children, Paris, France.; Imagine Institute, Paris University, Paris, France., Casanova JL; St Giles Laboratory of Human Genetics of Infectious Diseases, The Rockefeller University, New York, New York, USA.; Laboratory of Human Genetics of Infectious Diseases, Necker Branch, Inserm U1163, Necker Hospital for Sick Children, Paris, France.; Imagine Institute, Paris University, Paris, France.; Howard Hughes Medical Institute, New York, New York, USA.; Department of Pediatrics, Necker Hospital for Sick Children, Paris, France., Uitto J; Department of Dermatology and Cutaneous Biology, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania, USA., Vahidnezhad H; Center for Applied Genomics, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.; Division of Human Genetics, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.; Department of Pediatrics, Perelman School of Medicine, The University of Pennsylvania, Philadelphia, Pennsylvania, USA.; Department of Dermatology, Perelman School of Medicine, The University of Pennsylvania, Philadelphia, Pennsylvania, USA.
Jazyk:	angličtina
Zdroj:	JID innovations : skin science from molecules to population health [JID Innov] 2024 Mar 27; Vol. 4 (4), pp. 100278. Date of Electronic Publication: 2024 Mar 27 (Print Publication: 2024).
DOI:	10.1016/j.xjidi.2024.100278
Abstrakt:	Most viral infections can be self-limited, with no requirement for medical intervention. However, the same viruses can cause severe diseases in patients with compromised immunity due to single-gene diseases, acquired immune deficiency syndrome, or hematologic malignancies or those receiving immunosuppressive drugs. Occasionally, these immunocompromised patients harbor >1 infectious agent, requiring several concomitant diagnostic tests. We have developed, to our knowledge, a previously unreported whole-transcriptome sequencing-based pipeline that allows virome profiling, quantitation, and expression pattern analysis of 926 distinct viruses by sequencing of RNA isolated from a single lesional skin biopsy. This pipeline can also explore host genetics if there is a Mendelian predisposition to infection. We applied this pipeline to 6 Iranian patients with viral-induced skin lesions associated with immune deficiency secondary to HIV, human T-lymphotropic virus 1, chronic lymphocytic leukemia, and post transplant immunosuppression. In 5 cases, definitive human papillomavirus infections were identified, some caused by multiple viral types. In addition to human papillomavirus, coinfection with other viruses (Merkle cell polyomavirus, cytomegalovirus, and human herpesvirus 4) was detected in some lesions. In 1 case, whole-transcriptome sequencing validated the clinical diagnosis of adult T-cell leukemia/lymphoma in a patient with an initial diagnosis of mycosis fungoides/Sézary syndrome. These findings attest to the power of whole-transcriptome sequencing in profiling the cutaneous virome in the context of compromised immunity. (© 2024 The Authors.)
Databáze:	MEDLINE
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