Exploring the anxiolytic mechanism of Fructus gardeniae based on metabolomics, network pharmacology, and molecular docking.

Autor:	Tian Y; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Liang Xiang Town, Fangshan District, Beijing 102488, China., Yuan F; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Liang Xiang Town, Fangshan District, Beijing 102488, China., Kong J; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Liang Xiang Town, Fangshan District, Beijing 102488, China., Yuan Z; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Liang Xiang Town, Fangshan District, Beijing 102488, China., Jia C; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Liang Xiang Town, Fangshan District, Beijing 102488, China., Kui H; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Liang Xiang Town, Fangshan District, Beijing 102488, China., Yin Z; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Liang Xiang Town, Fangshan District, Beijing 102488, China., Liu C; Henan Key Laboratory of Rare Diseases, Endocrinology and Metabolism Center, The First Affiliated Hospital, College of Clinical Medicine of Henan University of Science and Technology, Luoyang 471003, China., Huang J; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Liang Xiang Town, Fangshan District, Beijing 102488, China.
Jazyk:	angličtina
Zdroj:	The Journal of pharmacy and pharmacology [J Pharm Pharmacol] 2024 Oct 03; Vol. 76 (10), pp. 1310-1327.
DOI:	10.1093/jpp/rgad102
Abstrakt:	Objective: To explore the effect and anxiolytic mechanism of a natural remedy called Fructus gardeniae (FG). Methods: The elevated-plus maze (EPM) test was used to confirm the anxiolytic effect of FG. The potential and anxiolytic components, targets, and route processes of FG were investigated using the network pharmacology method in conjunction with metabolomics and molecular docking technologies. Results: FG could greatly enhance the proportion of time and times of opening arms, according to the EPM data. As to the metabolomics findings, a total of 61 distinct metabolites were found, mainly involved in glycine, serine, and threonine metabolism as well as alanine, aspartate, and glutamate metabolism. The primary active ingredients of FG, nicotiflorin, jasminodiol, and crocetin, demonstrated substantial binding affinities with monoamine oxidase A (MAOA), monoamine oxidase A (ACHE), malate dehydrogenase 2 (MDH2), glutamate decarboxylase 2 (GAD2), glutamate decarboxylase 1 (GAD1), and nitric oxide synthase (NOS1), according to the findings of network pharmacology and molecular docking. Conclusion: FG exerts an anxiolytic action via targeting MAOA, ACHE, MDH2, GAD2, GAD1, and NOS1, and regulating the metabolism of glycine, serine, and threonine as well as alanine, aspartic acid, and glutamic acid. (© The Author(s) 2024. Published by Oxford University Press on behalf of the Royal Pharmaceutical Society.)
Databáze:	MEDLINE
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