Metagenomic Next-Generation Sequencing as an Effective Diagnostic Tool for Talaromycosis in HIV-Negative Patients.

Autor: Jiang L; Department of Dermatology and Venereology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China.; Fangchenggang Wanqing Institute of Mycosis Prevention and Control, Fangchenggang, China., Liang TW; Department of Dermatology and Venereology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China.; Fangchenggang Wanqing Institute of Mycosis Prevention and Control, Fangchenggang, China., Al-Odaini N; Department of Dermatology and Venereology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China.; Fangchenggang Wanqing Institute of Mycosis Prevention and Control, Fangchenggang, China., Hu Y; Richardson Medical Fungal Laboratory, Guangzhou Centre for Fungal Diagnostics and Research, Guangzhou, China., Huang M; Department of Ophthalmology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China., Wei L; Department of Dermatology and Venereology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China.; Fangchenggang Wanqing Institute of Mycosis Prevention and Control, Fangchenggang, China., Li XY; Department of Dermatology and Venereology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China.; Fangchenggang Wanqing Institute of Mycosis Prevention and Control, Fangchenggang, China., Pan KS; Department of Dermatology and Venereology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China.; Fangchenggang Wanqing Institute of Mycosis Prevention and Control, Fangchenggang, China., Zheng DY; Department of Dermatology and Venereology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China.; Fangchenggang Wanqing Institute of Mycosis Prevention and Control, Fangchenggang, China., Jiang ZW; Department of Dermatology and Venereology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China.; Fangchenggang Wanqing Institute of Mycosis Prevention and Control, Fangchenggang, China., Wei G; Department of Dermatology and Venereology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China. weigao126@126.com.; Fangchenggang Wanqing Institute of Mycosis Prevention and Control, Fangchenggang, China. weigao126@126.com., Cao CW; Department of Dermatology and Venereology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China. caocunwei@yeah.net.; Fangchenggang Wanqing Institute of Mycosis Prevention and Control, Fangchenggang, China. caocunwei@yeah.net.
Jazyk: angličtina
Zdroj: Mycopathologia [Mycopathologia] 2024 Jul 10; Vol. 189 (4), pp. 63. Date of Electronic Publication: 2024 Jul 10.
DOI: 10.1007/s11046-024-00866-x
Abstrakt: The diagnosis of Talaromyces marneffei infection in HIV-negative patients remains challenging. There is an urgent need for rapid and convenient methods to diagnose this complicated disease. The aim of this study was to evaluate the diagnostic efficiency of metagenomic next-generation sequencing (mNGS) for talaromycosis in non-HIV-infected patients by comparing mNGS with traditional microbial culture. In total, 66 samples from 57 patients were analyzed via both mNGS and microbial culture. The ROC curve showed a sensitivity for mNGS of 97.22%, which was greater than that of microbial culture (61.11%). Samples from the respiratory tract, infectious skin lesions, and lymph nodes are recommended as routine samples for talaromycosis detection via mNGS. Furthermore, mNGS significantly reduced the diagnostic time compared to microbial culture. Overall, our study demonstrated that mNGS is a promising tool for rapid and accurate pathogenic detection in HIV-negative patients with talaromycosis.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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