| Autor: |
Damm M; Institut für Chemie, Technische Universität Berlin, Straße des 17. Juni 135, 10623 Berlin, Germany.; LOEWE-Centre for Translational Biodiversity Genomics, Senckenberganlage 25, 60325 Frankfurt am Main, Germany.; Institute for Insect Biotechnology, Justus-Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Gießen, Germany., Karış M; Program of Laboratory Technology, Department of Chemistry and Chemical Process Technologies, Acıgöl Vocational School of Technical Sciences, Nevşehir Hacı Bektaş Veli University, Acıgöl, 50140 Nevşehir, Türkiye., Petras D; Department of Biochemistry, University of California Riverside, 169 Aberdeen Dr, Riverside, California 92507, United States.; Interfaculty Institute of Microbiology and Infection Medicine, University of Tuebingen, Auf der Morgenstelle 24, 72076 Tuebingen, Germany., Nalbantsoy A; Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, 35100 Izmir, Türkiye., Göçmen B; Zoology Section, Department of Biology, Faculty of Science, Ege University, Bornova, 35100 Izmir, Türkiye., Süssmuth RD; Institut für Chemie, Technische Universität Berlin, Straße des 17. Juni 135, 10623 Berlin, Germany. |
| Abstrakt: |
Snake venom variations are a crucial factor to understand the consequences of snakebite envenoming worldwide, and therefore it is important to know about toxin composition alterations between taxa. Palearctic vipers of the genera Vipera , Montivipera , Macrovipera, and Daboia have high medical impacts across the Old World. One hotspot for their occurrence and diversity is Türkiye, located on the border between continents, but many of their venoms remain still understudied. Here, we present the venom compositions of seven Turkish viper taxa. By complementary mass spectrometry-based bottom-up and top-down workflows, the venom profiles were investigated on proteomics and peptidomics level. This study includes the first venom descriptions of Vipera berus barani , Vipera darevskii , Montivipera bulgardaghica albizona, and Montivipera xanthina , as well as the first snake venomics profiles of Turkish Macrovipera lebetinus obtusa , and Daboia palaestinae , including an in-depth reanalysis of M. bulgardaghica bulgardaghica venom. Additionally, we identified the modular consensus sequence pEXW(PZ) 1-2 P(EI)/(KV)PPLE for bradykinin-potentiating peptides in viper venoms. For better insights into variations and potential impacts of medical significance, the venoms were compared against other Palearctic viper proteomes, including the first genus-wide Montivipera venom comparison. This will help the risk assessment of snakebite envenoming by these vipers and aid in predicting the venoms' pathophysiology and clinical treatments. |
