Quantified planar collagen distribution in healthy and degenerative mitral valve: biomechanical and clinical implications.

Autor: Sadeghinia MJ; Department of Structural Engineering, Norwegian University of Science and Technology (NTNU), Richard Birkelands Vei 1A, 7034, Trondheim, Norway., Persson RM; Department of Heart Disease, Haukeland University Hospital, Bergen, Norway.; Institute of Clinical Science, Medical Faculty, University of Bergen, Bergen, Norway., Ellensen VS; Department of Heart Disease, Haukeland University Hospital, Bergen, Norway., Haaverstad R; Department of Heart Disease, Haukeland University Hospital, Bergen, Norway.; Institute of Clinical Science, Medical Faculty, University of Bergen, Bergen, Norway., Holzapfel GA; Department of Structural Engineering, Norwegian University of Science and Technology (NTNU), Richard Birkelands Vei 1A, 7034, Trondheim, Norway.; Institute of Biomechanics, Graz University of Technology, Graz, Austria., Skallerud B; Department of Structural Engineering, Norwegian University of Science and Technology (NTNU), Richard Birkelands Vei 1A, 7034, Trondheim, Norway., Prot V; Department of Structural Engineering, Norwegian University of Science and Technology (NTNU), Richard Birkelands Vei 1A, 7034, Trondheim, Norway. victorien.prot@ntnu.no., Urheim S; Department of Heart Disease, Haukeland University Hospital, Bergen, Norway.; Institute of Clinical Science, Medical Faculty, University of Bergen, Bergen, Norway.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Jul 08; Vol. 14 (1), pp. 15670. Date of Electronic Publication: 2024 Jul 08.
DOI: 10.1038/s41598-024-65598-w
Abstrakt: Degenerative mitral valve disease is a common valvular disease with two arguably distinct phenotypes: fibroelastic deficiency and Barlow's disease. These phenotypes significantly alter the microstructures of the leaflets, particularly the collagen fibers, which are the main mechanical load carriers. The predominant method of investigation is histological sections. However, the sections are cut transmurally and provide a lateral view of the microstructure of the leaflet, while the mechanics and function are determined by the planar arrangement of the collagen fibers. This study, for the first time, quantitatively examined planar collagen distribution quantitatively in health and disease using second harmonic generation microscopy throughout the thickness of the mitral valve leaflets. Twenty diseased samples from eighteen patients and six control samples were included in this study. Healthy tissue had highly aligned collagen fibers. In fibroelastic deficiency they are less aligned and in Barlow's disease they are completely dispersed. In both diseases, collagen fibers have two preferred orientations, which, in contrast to the almost constant one orientation in healthy tissues, also vary across the thickness. The results indicate altered in vivo mechanical stresses and strains on the mitral valve leaflets as a result of disease-related collagen remodeling, which in turn triggers further remodeling.
(© 2024. The Author(s).)
Databáze: MEDLINE
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