Protection against α-Amanitin-induced liver toxicity: Efficacy of pomegranate seed oil and black cumin oil.

Autor: Sezer F; Department of Medical Pharmacology, Faculty of Medicine, Gazi University, Ankara, Turkey. Electronic address: fatihsezer58@gmail.com., Elmazoğlu Z; Ankara Medipol University, Faculty of Pharmacy, Ankara, Turkey. Electronic address: zubeyir.elmazoglu@ankaramedipol.edu.tr., Esendağlı G; Department of Pathology, Faculty of Medicine, Gazi University, Ankara, Turkey. Electronic address: guldalyilmaz@gazi.edu.tr., İlhan SÖ; Department of Medical Pharmacology, Faculty of Medicine, Gazi University, Ankara, Turkey. Electronic address: soilhan@gazi.edu.tr., Karasu Ç; Department of Medical Pharmacology, Faculty of Medicine, Gazi University, Ankara, Turkey. Electronic address: cimenkrs@gmail.com.
Jazyk: angličtina
Zdroj: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2024 Aug 28; Vol. 247, pp. 107854. Date of Electronic Publication: 2024 Jul 06.
DOI: 10.1016/j.toxicon.2024.107854
Abstrakt: The consumption of mushrooms containing α-Amanitin (α-A) can lead to severe liver damage. In this study, toxicological experiments were conducted to confirm the protective effects of pomegranate seed oil (PSO) and black cumin oil (BCO) against α-A-induced hepatotoxicity. Rats exposed once to α-A (3 mg/kg bw, i.p.) or saline alone (0.1 ml, i.p.) were either left untreated or treated with PSO or BCO at a dose of 2 ml/kg bw/day by oral gavage on the same day, and the treatment was continued for 7 days. Serum aminotransferases (ALT and AST), alkaline phosphatase (ALP) and total protein levels were measured and the active caspase 3 (cl-caspase 3) was evaluated by western blotting in the liver. Serum ALT, AST and ALP levels tended to decrease in the α-A exposed group, but no statistically significant difference was found compared to the saline group (p > 0.05). PSO and BCO did not affect serum liver function tests in rats exposed to saline or α-A. α-A toxicity was demonstrated by a significant decrease in serum total protein level (p < 0.05), a significant increase in liver cl-caspase 3 expression (p < 0.05), and structural liver damage mainly characterized by mononuclear inflammation and steatosis. When α-A exposed rats were treated with BCO, the increase in cl-caspase 3 was not inhibited, on the contrary BCO increased cl-caspase 3 in healthy rats (p < 0.05). PSO significantly ameliorated α-A-induced cl-caspase 3 increase and inflammatory histopathology in the liver. Both PSO and BCO completely prevented α-A-induced protein degradation. The findings indicate that PSO and BCO may protect liver functions against α-A-induced hepatotoxicity, encouraging future comprehensive studies to test them at different doses and frequency.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Ltd.)
Databáze: MEDLINE
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