Comparative Genomic and Genetic Evidence on a Role for the OarX Protein in Thiamin Salvage.
| Autor: | Oliveira-Filho ER; Horticultural Sciences Department, University of Florida, Gainesville, Florida 32611, United States., Rodionov DA; Infectious and Inflammatory Diseases Center, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, California 92037, United States., Hanson AD; Horticultural Sciences Department, University of Florida, Gainesville, Florida 32611, United States. |
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| Jazyk: | angličtina |
| Zdroj: | ACS omega [ACS Omega] 2024 Jun 21; Vol. 9 (26), pp. 28888-28894. Date of Electronic Publication: 2024 Jun 21 (Print Publication: 2024). |
| DOI: | 10.1021/acsomega.4c03514 |
| Abstrakt: | Salvage pathways for thiamin and its thiazole and pyrimidine moieties are poorly characterized compared to synthesis pathways. A candidate salvage gene is oarX , which encodes a short-chain dehydrogenase/reductase. In diverse bacteria, oarX clusters on the chromosome with genes of thiamin synthesis, salvage, or transport and is preceded by a thiamin pyrophosphate riboswitch. Thiamin and its moieties can undergo oxidations that convert a side-chain hydroxymethyl group to a carboxyl group, or the thiazole ring to a thiazolone, causing a loss of biological activity. To test if OarX participates in salvage of the carboxyl or thiazolone products, we used a genetic approach in Corynebacterium glutamicum ATCC 14067, which is auxotrophic for thiamin's pyrimidine moiety. This strain could not utilize the pyrimidine carboxyl derivative. This excluded a role in salvaging this product and narrowed the function search to metabolism of the carboxyl or thiazolone derivatives of thiamin or its thiazole moiety. However, a Δ thiG (thiazole auxotroph) strain was not rescued by any of these derivatives. Nor did deleting oarX affect rescue by the physiological pyrimidine and thiazole precursors of thiamin. These findings reinforce the genomic evidence that OarX has a function in thiamin metabolism and rule out five logical possibilities for what this function is. Competing Interests: The authors declare no competing financial interest. (© 2024 The Authors. Published by American Chemical Society.) |
| Databáze: | MEDLINE |
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