Advances and challenges in investigating B-cells via single-cell transcriptomics.

Autor: Skinner OP; Department of Microbiology & Immunology, University of Melbourne, Peter Doherty Institute for Infection and Immunity, 792 Elizabeth Street, Parkville, Melbourne, VIC 3000, Australia. Electronic address: ollie.skinner@unimelb.edu.au., Asad S; Department of Microbiology & Immunology, University of Melbourne, Peter Doherty Institute for Infection and Immunity, 792 Elizabeth Street, Parkville, Melbourne, VIC 3000, Australia., Haque A; Department of Microbiology & Immunology, University of Melbourne, Peter Doherty Institute for Infection and Immunity, 792 Elizabeth Street, Parkville, Melbourne, VIC 3000, Australia. Electronic address: ashraful.haque@unimelb.edu.au.
Jazyk: angličtina
Zdroj: Current opinion in immunology [Curr Opin Immunol] 2024 Jun; Vol. 88, pp. 102443. Date of Electronic Publication: 2024 Jul 04.
DOI: 10.1016/j.coi.2024.102443
Abstrakt: Single-cell RNA sequencing (scRNAseq) and Variable, Diversity, Joining (VDJ) profiling have improved our understanding of B-cells. Recent scRNAseq-based approaches have led to the discovery of intermediate B-cell states, including preplasma cells and pregerminal centre B-cells, as well as unveiling protective roles for B-cells within tertiary lymphoid structures in respiratory infections and cancers. These studies have improved our understanding of transcriptional and epigenetic control of B-cell development and of atypical and memory B-cell differentiation. Advancements in temporal profiling in parallel with transcriptomic and VDJ sequencing have consolidated our understanding of the trajectory of B-cell clones over the course of infection and vaccination. Challenges remain in studying B-cell states across tissues in humans, in relating spatial location with B-cell phenotype and function, in examining antibody isotype switching events, and in unequivocal determination of clonal relationships. Nevertheless, ongoing multiomic assessments and studies of cellular interactions within tissues promise new avenues for improving humoral immunity and combatting autoimmune conditions.
Competing Interests: Declaration of Competing Interest The authors A/Prof Ashraful Haque, Saba Asad and Dr. Oliver P Skinner have no conflicts of interest to declare in the submission of this manuscript.
(Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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