Protocol for adeno-associated virus-mediated optogenetic activation of olfactory output neurons in neonatal mice.
| Autor: | Chen YN; Institute of Developmental Neurophysiology, Center of Molecular Neurobiology, Hamburg Center of Neuroscience, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany. Electronic address: yunan.chen@zmnh.uni-hamburg.de., Kostka JK; Institute of Developmental Neurophysiology, Center of Molecular Neurobiology, Hamburg Center of Neuroscience, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany., Bitzenhofer SH; Institute of Developmental Neurophysiology, Center of Molecular Neurobiology, Hamburg Center of Neuroscience, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany., Hanganu-Opatz IL; Institute of Developmental Neurophysiology, Center of Molecular Neurobiology, Hamburg Center of Neuroscience, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany. Electronic address: hangop@zmnh.uni-hamburg.de. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2024 Sep 20; Vol. 5 (3), pp. 103164. Date of Electronic Publication: 2024 Jul 04. |
| DOI: | 10.1016/j.xpro.2024.103164 |
| Abstrakt: | Optogenetic manipulation has proven a powerful tool for investigating the mechanisms underlying the function of neuronal networks, but implementing the technique on mammals during early development remains challenging. Here, we present a comprehensive workflow to specifically manipulate mitral/tufted cells (M/TCs), the output neurons in the olfactory circuit, mediated by adeno-associated virus (AAV) transduction and light stimulation in neonatal mice and monitor neuronal and network activity with in vivo electrophysiology. This method represents an efficient approach to elucidate functional brain development. For complete details on the use and execution of this protocol, please refer to Chen et al. 1 , 2 , 3 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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