Complete photodynamic inactivation of Pseudomonas aeruginosa biofilm with use of potassium iodide and its comparison with enzymatic pretreatment.

Autor: Alves F; São Carlos Institute of Physics (IFSC), University of São Paulo (USP), Brazil, Av. Trabalhador São-carlense, 400 - Centro, CEP 13560-970 São Carlos, SP, Brazil., Nakada PJT; São Carlos Institute of Physics (IFSC), University of São Paulo (USP), Brazil, Av. Trabalhador São-carlense, 400 - Centro, CEP 13560-970 São Carlos, SP, Brazil., Marques MJAM; São Carlos Institute of Physics (IFSC), University of São Paulo (USP), Brazil, Av. Trabalhador São-carlense, 400 - Centro, CEP 13560-970 São Carlos, SP, Brazil., Rea LDC; São Carlos Institute of Physics (IFSC), University of São Paulo (USP), Brazil, Av. Trabalhador São-carlense, 400 - Centro, CEP 13560-970 São Carlos, SP, Brazil., Cortez AA; São Carlos Institute of Physics (IFSC), University of São Paulo (USP), Brazil, Av. Trabalhador São-carlense, 400 - Centro, CEP 13560-970 São Carlos, SP, Brazil., Pellegrini VOA; São Carlos Institute of Physics (IFSC), University of São Paulo (USP), Brazil, Av. Trabalhador São-carlense, 400 - Centro, CEP 13560-970 São Carlos, SP, Brazil., Polikarpov I; São Carlos Institute of Physics (IFSC), University of São Paulo (USP), Brazil, Av. Trabalhador São-carlense, 400 - Centro, CEP 13560-970 São Carlos, SP, Brazil., Kurachi C; São Carlos Institute of Physics (IFSC), University of São Paulo (USP), Brazil, Av. Trabalhador São-carlense, 400 - Centro, CEP 13560-970 São Carlos, SP, Brazil. Electronic address: cristina@ifsc.usp.br.
Jazyk: angličtina
Zdroj: Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2024 Aug; Vol. 257, pp. 112974. Date of Electronic Publication: 2024 Jun 29.
DOI: 10.1016/j.jphotobiol.2024.112974
Abstrakt: Pseudomonas aeruginosa, a gram-negative bacterium, accounts for 7% of all hospital-acquired infections. Despite advances in medicine and antibiotic therapy, P. aeruginosa infection still results in high mortality rates of up to 62% in certain patient groups. This bacteria is also known to form biofilms, that are 10 to 1000 times more resistant to antibiotics compared to their free-floating counterparts. Photodynamic Inactivation (PDI) has been proved to be an effective antimicrobial technique for microbial control. This method involves the incubation of the pathogen with a photosensitizer (PS), then, a light at appropriated wavelength is applied, leading to the production of reactive oxygen species that are toxic to the microbial cells. Studies have focused on strategies to enhance the PDI efficacy, such as a pre-treatment with enzymes to degrade the biofilm matrix and/or an addition of inorganic salts to the PS. The aim of the present study is to evaluate the effectiveness of PDI against P. aeruginosa biofilm in association with the application of the enzymes prior to PDI (enzymatic pre-treatment) or the addition of potassium iodide (KI) to the photosensitizer solution, to increase the inactivation effectiveness of the treatment. First, a range of enzymes and PSs were tested, and the best protocols for combined treatments were selected. The results showed that the use of enzymes as a pre-treatment was effective to reduce the total biomass, however, when associated with PDI, mild bacterial reductions were obtained. Then, the use of KI in association with the PS was evaluated and the results showed that, PDI mediated by methylene blue (MB) in the presence of KI was able to completely eradicate the biofilm. However, when the PDI was performed with curcumin and KI, no additive reduction was observed. In conclusion, out of all strategies evaluated in the present study, the most promising strategy to improve PDI against P. aeruginosa biofilm was the use of KI in association with MB, resulting in eradication with 10 8 log bacterial inactivation.
Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests. Cristina Kurachi reports financial support was provided by The São Paulo Research Foundation. Cristina Kurachi reports financial support was provided by National Council for Scientific and Technological Development. Fernanda Alves reports financial support was provided by The São Paulo Research Foundation. Maria Julia de Arruda Mazzotti Marques reports financial support was provided by Coordination for the Improvement of Higher Education Personnel. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. The authors of the manuscript “Complete photodynamic inactivation of Pseudomonas aeruginosa biofilm with use of potassium iodide and its comparison with enzymatic pretreatment” by F. Alves et al., does not have any conflict of interest.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: