Streamlining large-scale oceanic biomonitoring using passive eDNA samplers integrated into vessel's continuous pump underway seawater systems.

Autor: Jeunen GJ; Department of Marine Science, University of Otago, Dunedin 9016, New Zealand. Electronic address: gjeunen@gmail.com., Mills S; National Institute of Water and Atmospheric Research, Wellington 6021, New Zealand., Mariani S; School of Biological and Environmental Sciences, Liverpool John Moores University, Liverpool L3 3AF, UK., Treece J; Department of Anatomy, School of Biomedical Sciences, University of Otago, Dunedin 9016, New Zealand., Ferreira S; Department of Anatomy, School of Biomedical Sciences, University of Otago, Dunedin 9016, New Zealand., Stanton JL; Department of Anatomy, School of Biomedical Sciences, University of Otago, Dunedin 9016, New Zealand., Durán-Vinet B; Department of Anatomy, School of Biomedical Sciences, University of Otago, Dunedin 9016, New Zealand., Duffy GA; Department of Marine Science, University of Otago, Dunedin 9016, New Zealand., Gemmell NJ; Department of Anatomy, School of Biomedical Sciences, University of Otago, Dunedin 9016, New Zealand., Lamare M; Department of Marine Science, University of Otago, Dunedin 9016, New Zealand.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2024 Oct 10; Vol. 946, pp. 174354. Date of Electronic Publication: 2024 Jun 30.
DOI: 10.1016/j.scitotenv.2024.174354
Abstrakt: Passive samplers are enabling the scaling of environmental DNA (eDNA) biomonitoring in our oceans, by circumventing the time-consuming process of water filtration. Designing a novel passive sampler that does not require extensive sample handling time and can be connected to ocean-going vessels without impeding normal underway activities has potential to rapidly upscale global biomonitoring efforts onboard the world's oceanic fleet. Here, we demonstrate the utility of an artificial sponge sampler connected to the continuous pump underway seawater system as a means to enable oceanic biomonitoring. We compared the performance of this passive sampling protocol with standard water filtration at six locations during a research voyage from New Zealand to Antarctica in early 2023. Eukaryote metabarcoding of the mitochondrial COI gene revealed no significant difference in phylogenetic α-diversity between sampling methods and both methods delineated a progressive reduction in number of Zero-Radius Operational Taxonomic Units (ZOTUs) with increased latitudes. While both sampling methods revealed comparable trends in geographical community compositions, distinct clusters were identified for passive samplers and water filtration at each location. Additionally, greater variability between replicates was observed for passive samplers, resulting in an increased estimated level of replication needed to recover 90 % of the biodiversity. Furthermore, traditional water filtration failed to detect three phyla observed by passive samplers and extrapolation analysis estimated passive samplers recover a larger number of ZOTUs compared to water filtration for all six locations. Our results demonstrate the potential of this passive eDNA sampler protocol and highlight areas where this emerging technology could be improved, thereby enabling large-scale offshore marine eDNA biomonitoring by leveraging the world's oceanic fleet without interfering with onboard activities.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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