Pyrobacteriopheophorbide-a derivatives possessing various hydrophilic esterifying groups at the C17-propionate residues for photodynamic therapy.

Autor: Sasaki SI; Nagahama Institute of Bio-Science and Technology, Nagahama, Shiga, Japan.; Graduate School of Life Sciences, Ritsumeikan University, Kusatsu, Shiga, Japan., Morioka Y; Nagahama Institute of Bio-Science and Technology, Nagahama, Shiga, Japan., Maegawa K; Nagahama Institute of Bio-Science and Technology, Nagahama, Shiga, Japan., Katsuragi Y; Nagahama Institute of Bio-Science and Technology, Nagahama, Shiga, Japan., Nakamura T; Nagahama Institute of Bio-Science and Technology, Nagahama, Shiga, Japan., Kamemura K; Nagahama Institute of Bio-Science and Technology, Nagahama, Shiga, Japan., Tamiaki H; Graduate School of Life Sciences, Ritsumeikan University, Kusatsu, Shiga, Japan.
Jazyk: angličtina
Zdroj: Photochemistry and photobiology [Photochem Photobiol] 2024 Jul 02. Date of Electronic Publication: 2024 Jul 02.
DOI: 10.1111/php.13995
Abstrakt: Aiming at the application to photodynamic therapy, natural bacteriochlorophyll-a was converted to chemically stable free-base derivatives possessing different kinds of hydrophilic C17-propionate residues. These semi-synthetic bacteriochlorins were found to have self-assembling ability in an aqueous environment and formed stable J-type aggregates in a cell culture medium containing 0.2% DMSO. The electronic absorption spectra of all the sensitizers showed Q y absorption maxima at 754 nm in DMSO as their monomeric states, while a drastic shift of the red-most bands to ca. 880 nm was observed in the aqueous medium. The circular dichroism spectra in the medium showed much intense signals compared to those measured in DMSO, supporting the formation of well-ordered supramolecular structures. By introducing hydrophilic side chains, the bacteriochlorin sensitizers could be dispersed in the aqueous medium as their J-aggregates without the use of any surfactants. Cellular uptake efficiencies as well as photodynamic activities were evaluated using human cervical adenocarcinoma HeLa cells. Among the 11 photosensitizers investigated, the best result was obtained for a charged derivative possessing trimethylammonium terminal (17-CH 2 CH 2 COOCH 2 CH 2 N + (CH 3 ) 3 I - ) and photocytotoxicity of EC 50  = 0.09 μM was achieved by far-red light illumination of 35 J/cm 2 from an LED panel (730 nm).
(© 2024 American Society for Photobiology.)
Databáze: MEDLINE
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