| Autor: |
Zhang X; Department of Gastroenterology, The Second Affiliated Hospital of Hebei North University,Zhangjiakou,Hebei 075100,China., Jia HY; Department of Clinical Laboratory, The Second Affiliated Hospital of Hebei North University,Zhangjiakou,Hebei 075100,China., Song WX; Department of Radiology,Zhangjiakou Xuangang Hospital,Zhangjiakou,Hebei 075100,China., Zhao HQ; Department of Traditional Chinese Medicine,The Second Affiliated Hospital of Hebei North University,Zhangjiakou,Hebei 075100,China. |
| Jazyk: |
čínština |
| Zdroj: |
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae [Zhongguo Yi Xue Ke Xue Yuan Xue Bao] 2024 Jun; Vol. 46 (3), pp. 334-340. |
| DOI: |
10.3881/j.issn.1000-503X.15863 |
| Abstrakt: |
Objective To explore the relationship between the expression levels of microRNA-155 (miR-155) and suppressor of cytokine signaling 1 (SOCS1) in the colonic mucosal tissue of patients with ulcerative colitis (UC) and the severity of the disease.Methods A total of 130 UC patients admitted to the Second Affiliated Hospital of Hebei North University from September 2021 to June 2023 were selected.According to the modified Mayo score system,the patients were assigned into an active stage group ( n =85) and a remission stage group ( n =45).According to the modified Truelove and Witts classification criteria,the UC patients at the active stage were assigned into a mild group ( n =35),a moderate group ( n =30),and a severe group ( n =20).A total of 90 healthy individuals who underwent colonoscopy for physical examination or those who had normal colonoscopy results after single polypectomy and excluded other diseases were selected as the control group.The colonic mucosal tissues of UC patients with obvious lesions and the colonic mucosal tissue 20 cm away from the anus of the control group were collected.The levels of miR-155 and SOCS1 mRNA in tissues were determined by fluorescence quantitative PCR,and the expression of SOCS1 protein in tissues was determined by immunohistochemistry.The correlations of the levels of miR-155 and SOCS1 mRNA in the colonic mucosal tissue with the modified Mayo score of UC patients were analyzed.The values of the levels of miR-155 and SOCS1 mRNA in predicting the occurrence of severe illness in the UC patients at the active stage were evaluated.Results Compared with the control group and the remission stage group,the active stage group showed up-regulated expression level of miR-155,down-regulated level of SOCS1 mRNA,and decreased positive rate of SOCS1 protein in the colonic mucosal tissue (all P <0.001).The expression level of miR-155 and modified Mayo score in colonic mucosal tissues of UC patients at the active stage increased,while the mRNA level of SOCS1 was down-regulated as the disease evolved from being mild to severe (all P <0.001).The modified Mayo score was positively correlated with the miR-155 level and negative correlated with the mRNA level of SOCS1 in colonic mucosal tissues of UC patients (all P <0.001).The high miR-155 level ( OR =2.762,95% CI =1.284-5.944, P =0.009),low mRNA level of SOCS1 ( OR =2.617,95% CI =1.302-5.258, P =0.007),and modified Mayo score≥12 points ( OR =3.232,95% CI =1.450-7.204, P =0.004) were all risk factors for severe disease in the UC patients at the active stage.The area under curve of miR-155 combined with SOCS1 mRNA in predicting severe illness in the UC patients at the active stage was 0.920.Conclusions The expression levels of miR-155 and SOCS1 mRNA were correlated with the disease severity in the UC patients at the active stage.The combination of the two indicators demonstrates good performance in predicting the occurrence of severe illness in UC patients at the active stage. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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