Extracellular vesicles in cattle infected with bovine leukaemia virus: isolation and molecular analysis.
| Autor: | Szczotka M; Department of Biochemistry, 24-100 Puławy, Poland., Wasiak M; Department of Pathology National Veterinary Research Institute, 24-100 Puławy, Poland., Kuźmak J; Department of Biochemistry, 24-100 Puławy, Poland. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of veterinary research [J Vet Res] 2024 May 27; Vol. 68 (2), pp. 189-198. Date of Electronic Publication: 2024 May 27 (Print Publication: 2024). |
| DOI: | 10.2478/jvetres-2024-0031 |
| Abstrakt: | Introduction: Exosomes are nanosized lipid bilayer membranous microvesicles, extracellularly released from a variety of mammalian cells. They mediate intercellular signalling by transporting several types of RNA, lipids and proteins and participate in the intercellular exchange of DNA, RNA, micro RNA, proteins and other components. These microvesicles are present in all body fluids in physiological and pathological conditions and reflect the state of the host organism. The aim of the study was the isolation and molecular determination of exosomes in blood and supernatant fluids of bovine dendritic cell cultures infected with bovine leukaemia virus (BLV). Material and Methods: Exosomes were isolated by ultracentrifugation from the blood sera, plasma and supernatant of bovine BLV-infected and uninfected control dendritic cell cultures and their presence was confirmed with scanning electron and transmission electron microscopy. Western blot analysis of the structural BLV glycoprotein 51 (Env) and protein 24 (Gag) and of the tetraspanin exosomal markers CD9, CD63 and flotillin-1 was undertaken in BLV+ and control BLV- cattle. Results: In exosomes of leukaemic cattle both BLV proteins and exosomal markers were detected. In healthy control animals only exosomal markers were determined. Conclusion: Proteins of BLV were released with exosomes and could be transferred into recipient cells as an alternative propagation route not requiring virus infection. Competing Interests: Conflict of Interests Statement The authors declare that there is no conflict of interests regarding the publication of this article. (© 2024 Maria Szczotka et al., published by Sciendo.) |
| Databáze: | MEDLINE |
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