Whole genome-based antimicrobial resistance and virulence profiling of Staphylococcus aureus isolates from chronic leg ulcer patients in Kilimanjaro, Tanzania.

Autor: Omar OS; General Surgery Department, Kilimanjaro Christian Medical Centre, P.O Box 3010, Moshi, Tanzania. Electronic address: dromarsaid2005@gmail.com., Sengeruan LP; Kishapu District Council, P.O. Box 1288, Shinyanga, Tanzania; Bioinformatics Unit, Kilimanjaro Clinical Research Institute, P.O. Box 2236, Moshi, Tanzania. Electronic address: lameckpashet@gmail.com., Kanje LE; Genomics Unit, Kilimanjaro Clinical Research Institute, P.O. Box 2236, Moshi, Tanzania., van Zwetselaar M; Bioinformatics Unit, Kilimanjaro Clinical Research Institute, P.O. Box 2236, Moshi, Tanzania., Kuchaka DJ; Bioinformatics Unit, Kilimanjaro Clinical Research Institute, P.O. Box 2236, Moshi, Tanzania; Department of Global Health and Biomedical Sciences, The Nelson Mandela African Institution of Science and Technology, P. O. Box 447, Arusha, Tanzania., Shayo MJ; Genomics Unit, Kilimanjaro Clinical Research Institute, P.O. Box 2236, Moshi, Tanzania; Department of Biological and Pre-clinical Studies, Muhimbili University of Health and Allied Sciences, P.O Box 65001, Dar es Salaam, Tanzania., Kumburu H; Genomics Unit, Kilimanjaro Clinical Research Institute, P.O. Box 2236, Moshi, Tanzania., Sonda T; Bioinformatics Unit, Kilimanjaro Clinical Research Institute, P.O. Box 2236, Moshi, Tanzania., Mshana J; General Surgery Department, Kilimanjaro Christian Medical Centre, P.O Box 3010, Moshi, Tanzania., Chugulu S; General Surgery Department, Kilimanjaro Christian Medical Centre, P.O Box 3010, Moshi, Tanzania.
Jazyk: angličtina
Zdroj: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [Infect Genet Evol] 2024 Sep; Vol. 123, pp. 105631. Date of Electronic Publication: 2024 Jun 28.
DOI: 10.1016/j.meegid.2024.105631
Abstrakt: Background: Chronic leg ulcers are hard to treat and can be a burden, particularly in resource-limited settings where diagnosis is a challenge. Staphylococcus aureus is among the common bacteria isolated from chronic wounds with a great impact on wound healing, particularly in patients with co-morbidities. Antimicrobial resistance genes and virulence factors in Staphylococcus aureus isolates were assessed to support healthcare professionals to make better therapeutic choices, and importantly to curb the development and spread of antibiotic resistance.
Methods: A cross-sectional study involved both inpatients and outpatients with chronic leg ulcers was conducted from August 2022 to April 2023 in 2 health facilities in Kilimanjaro region in Tanzania. Antimicrobial susceptibility testing was done using the disk diffusion method. Further, whole genome sequencing was performed to study the genotypic characteristics of the isolates.
Results: A total of 92 participants were recruited in which 9 participants were only positive for 10 Staphylococcus aureus isolates upon culture. Five STs among 9 isolates were identified. Most of them belonged to ST8 (44%), with 1 isolate does not belong to any ST. Additionally, 50% of the isolates were methicillin-resistant Staphylococcus aureus (MRSA). All S. aureus isolates had almost similar virulence factors such as hemolysin, proteases and evasions that promote toxin production, protease production and host immune evasion respectively. Moreover, all mecA positive S. aureus isolates were phenotypically susceptible to cefoxitin.
Conclusion: Presence of mecA positive S. aureus isolates which are also phenotypically susceptible to cefoxitin implies the possibility of classifying MRSA as MSSA. This may result in the possible emergence of highly cefoxitin - resistant strains in health care and community settings when subsequently exposed to beta-lactam agents. Therefore, combination of whole genome sequencing and conventional methods is important in assessing bacterial resistance and virulence to improve management of patients.
Competing Interests: Declaration of competing interest The authors declare that they have no competing interests.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
Externí odkaz: