European flow cytometry quality assurance guidelines for the diagnosis of primary immune deficiencies and assessment of immune reconstitution following B cell depletion therapies and transplantation.

Autor:	Kelleher P; Immunology of Infection, Department of Infectious Disease, Imperial College London, London, UK.; Department of Infection and Immunity Sciences, North West London Pathology, London, UK., Greathead L; Department of Infection and Immunity Sciences, North West London Pathology, London, UK., Whitby L; UK NEQAS for Leucocyte Immunophenotyping, Sheffield Teaching Hospitals NHS Foundation Trust, Sheffield, UK., Brando B; Hematology Laboratory and Transfusion Center, New Hospital of Legnano: Ospedale Nuovo di Legnano, Milan, Italy., Barnett D; UK NEQAS for Leucocyte Immunophenotyping, Sheffield Teaching Hospitals NHS Foundation Trust, Sheffield, UK., Bloxham D; Haematopathology and Oncology Diagnostic Service, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK., deTute R; Haematological Malignancy Diagnostic Service, St James's University Hospital, Leeds, UK., Dunlop A; Department of Haemato-Oncology, Royal Marsden Hospital, London, UK., Farren T; Division of Haemato-Oncology, St Bartholomew's Hospital, Barts Health NHS Trust, London, UK.; Pathology Group, Blizard Institute, Queen Mary University of London, London, UK., Francis S; Department of Haematology, Sheffield Teaching Hospitals NHS Foundation Trust, Sheffield, UK., Payne D; Tees Valley Pathology Service, James Cook University Hospital, Middlesbrough, UK., Scott S; UK NEQAS for Leucocyte Immunophenotyping, Sheffield Teaching Hospitals NHS Foundation Trust, Sheffield, UK., Snowden JA; Department of Haematology, Sheffield Teaching Hospitals NHS Foundation Trust, Sheffield, UK., Sorour Y; Haematology, Doncaster and Bassetlaw Teaching Hospitals NHS Trust, Doncaster, UK., Stansfield E; Greater Manchester Immunology Service, Manchester University NHS Foundation Trust, Manchester, UK., Virgo P; Department of Immunology and Immunogenetics, North Bristol NHS Trust, Bristol, UK., Whitby A; UK NEQAS for Leucocyte Immunophenotyping, Sheffield Teaching Hospitals NHS Foundation Trust, Sheffield, UK.
Jazyk:	angličtina
Zdroj:	Cytometry. Part B, Clinical cytometry [Cytometry B Clin Cytom] 2024 Nov; Vol. 106 (6), pp. 424-436. Date of Electronic Publication: 2024 Jun 28.
DOI:	10.1002/cyto.b.22195
Abstrakt:	Over the last 15 years activity of diagnostic flow cytometry services have evolved from monitoring of CD4 T cell subsets in HIV-1 infection to screening for primary and secondary immune deficiencies syndromes and assessment of immune constitution following B cell depleting therapy and transplantation. Changes in laboratory activity in high income countries have been driven by initiation of anti-retroviral therapy (ART) in HIV-1 regardless of CD4 T cell counts, increasing recognition of primary immune deficiency syndromes and the wider application of B cell depleting therapy and transplantation in clinical practice. Laboratories should use their experience in standardization and quality assurance of CD4 T cell counting in HIV-1 infection to provide immune monitoring services to patients with primary and secondary immune deficiencies. Assessment of immune reconstitution post B cell depleting agents and transplantation can also draw on the expertise acquired by flow cytometry laboratories for detection of CD34 stem cell and assessment of MRD in hematological malignancies. This guideline provides recommendations for clinical laboratories on providing flow cytometry services in screening for immune deficiencies and its emerging role immune reconstitution after B cell targeting therapies and transplantation. (© 2024 The Author(s). Cytometry Part B: Clinical Cytometry published by Wiley Periodicals LLC on behalf of International Clinical Cytometry Society.)
Databáze:	MEDLINE
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