| Autor: |
Dracheva KV; Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre 'Kurchatov Institute', 188300 Gatchina, Russia.; Department of Molecular-Genetic and Nanobiological Technologies, Scientific Research Center, Pavlov First Saint Petersburg State Medical University, 197022 St.-Petersburg, Russia., Pobozheva IA; Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre 'Kurchatov Institute', 188300 Gatchina, Russia.; Department of Molecular-Genetic and Nanobiological Technologies, Scientific Research Center, Pavlov First Saint Petersburg State Medical University, 197022 St.-Petersburg, Russia., Anisimova KA; Center for Surgical Treatment of Obesity and Metabolic Disorders, Pavlov First Saint Petersburg State Medical University, 197022 St.-Petersburg, Russia., Panteleeva AA; Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre 'Kurchatov Institute', 188300 Gatchina, Russia.; Department of Molecular-Genetic and Nanobiological Technologies, Scientific Research Center, Pavlov First Saint Petersburg State Medical University, 197022 St.-Petersburg, Russia., Garaeva LA; Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre 'Kurchatov Institute', 188300 Gatchina, Russia., Balandov SG; Center for Surgical Treatment of Obesity and Metabolic Disorders, Pavlov First Saint Petersburg State Medical University, 197022 St.-Petersburg, Russia., Hamid ZM; Center for Surgical Treatment of Obesity and Metabolic Disorders, Pavlov First Saint Petersburg State Medical University, 197022 St.-Petersburg, Russia., Vasilevsky DI; Center for Surgical Treatment of Obesity and Metabolic Disorders, Pavlov First Saint Petersburg State Medical University, 197022 St.-Petersburg, Russia., Pchelina SN; Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre 'Kurchatov Institute', 188300 Gatchina, Russia.; Department of Molecular-Genetic and Nanobiological Technologies, Scientific Research Center, Pavlov First Saint Petersburg State Medical University, 197022 St.-Petersburg, Russia.; Federal State Budgetary Research Institution 'Institute of Experimental Medicine', 197022 St.-Petersburg, Russia., Miroshnikova VV; Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre 'Kurchatov Institute', 188300 Gatchina, Russia.; Department of Molecular-Genetic and Nanobiological Technologies, Scientific Research Center, Pavlov First Saint Petersburg State Medical University, 197022 St.-Petersburg, Russia. |
| Abstrakt: |
Obesity is a risk factor for type 2 diabetes mellitus (T2DM) and cardiovascular disease (CVD). Adipose tissue (AT) extracellular vesicles (EVs) could play a role in obesity and T2DM associated CVD progression via the influence of their specific cargo on gene expression in recipient cells. The aim of this work was to evaluate the effects of AT EVs of patients with obesity with/without T2DM on reverse cholesterol transport (RCT)-related gene expression in human monocyte-derived macrophages (MDMs) from healthy donors. AT EVs were obtained after ex vivo cultivation of visceral and subcutaneous AT (VAT and SAT, respectively). ABCA1 , ABCG1 , PPARG , LXRβ (NR1H2) , and LXRα (NR1H3) mRNA levels in MDMs as well as in origine AT were determined by a real-time PCR. T2DM VAT and SAT EVs induced ABCG1 gene expression whereas LXRα and PPARG mRNA levels were simultaneously downregulated. PPARG mRNA levels also decreased in the presence of VAT EVs of obese patients without T2DM. In contrast ABCA1 and LXRβ mRNA levels tended to increase with the addition of obese AT EVs. Thus, AT EVs can influence RCT gene expression in MDMs during obesity, and the effects are dependent on T2DM status. |
