Effects of natural mating, artificial insemination and intravaginal deposition of raw semen or seminal plasma on vaginal and uterine blood flow in German Holstein cows.

Autor: Elmetwally MA; Institute for Reproductive Biology, University of Veterinary Medicine Hannover, 30559, Hannover, Germany.; Department of Theriogenology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.; Center for Reproductive Biotechnology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt., Meinecke-Tillmann S; Institute for Reproductive Biology, University of Veterinary Medicine Hannover, 30559, Hannover, Germany., Herzog K; Clinic for Cattle, University of Veterinary Medicine Hannover, 30173, Hannover, Germany.; Lower Saxony State Office for Consumer Protection and Food Safety, D-26203, Oldenburg, Germany., Bollwein H; Clinic for Cattle, University of Veterinary Medicine Hannover, 30173, Hannover, Germany. hbollwein@vetclinics.uzh.ch.; Clinic of Reproductive Medicine, Vetsuisse Faculty, University of Zürich, Zürich, CH-8057, Switzerland. hbollwein@vetclinics.uzh.ch.
Jazyk: angličtina
Zdroj: BMC veterinary research [BMC Vet Res] 2024 Jun 26; Vol. 20 (1), pp. 277. Date of Electronic Publication: 2024 Jun 26.
DOI: 10.1186/s12917-024-03919-x
Abstrakt: Aim: The present study was performed to characterize and compare the perfusion of vaginal and uterine arteries after challenging the reproductive tract of dairy cows via natural mating, artificial insemination (AI), or intravaginal deposition (vaginal fundus) of different biological fluids or a placebo.
Materials and Methods: In a double-blind study, six German Holstein cows were administered PGF 2α during dioestrus and 48 h later treated with GnRH. Intravaginal or intrauterine treatments were carried out 12 h after GnRH was administered. Animals served as their controls, using a cross-over design with an interval of 14 days between experiments. The experimental animals were allocated to receive the following treatments: natural mating (N), intrauterine artificial insemination (A), intravaginal deposition (vaginal fundus) of 6 mL raw semen (R) or 6 mL seminal plasma (S), and compared to their controls [control 1: 6 mL placebo (P: physiological saline); control 2: no treatment (C)). Corresponding time intervals were chosen for the untreated control oestrus. Blood flow volume (BFV) in the uterine (u) and vaginal (v) arteries ipsilateral to the ovary bearing the preovulatory follicle was determined using transrectal Doppler sonography.
Results: All animals exhibited oestrus and ovulated between 30 and 36 h after GnRH. Transient increases (P < 0.05) in vaginal blood flow occurred between 3 and 12 h following mating as well as 3 to 9 h after deposition of raw semen and seminal plasma, respectively. The most distinct increases (199%) in vBFV occurred 6 h after mating compared to values immediately before mating (= time 0 h). Neither AI nor deposition of a placebo into the vagina affected vBFV (P > 0.05). Only mating and deposition of either raw semen, seminal plasma or AI increased uBFV (P < 0.003). The greatest rise in uBFV occurred after natural mating. Maximum uBFV values were detected 9 h after mating when values were 79% greater (P < 0.05) than at 0 h.
Conclusions: The natural mating, deposition of raw semen or seminal plasma and conventional AI affect vaginal and/or uterine blood flow to different degrees. The factors responsible for these alterations in blood flow and their effects on fertility remain to be clarified in future studies.
(© 2024. The Author(s).)
Databáze: MEDLINE
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