Surface Engineering for Endothelium-Mimicking Functions to Combat Infection and Thrombosis in Extracorporeal Life Support Technologies.

Autor: Ashcraft M; Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia, Athens, Georgia, 30602, USA., Garren M; School of Chemical, Materials and Biomedical Engineering, College of Engineering, University of Georgia, Athens, Georgia, 30602, USA., Lautner-Csorba O; Department of Surgery, University of Michigan Medical Center, Ann Arbor, Michigan, 48109, USA., Pinon V; Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia, Athens, Georgia, 30602, USA., Wu Y; School of Chemical, Materials and Biomedical Engineering, College of Engineering, University of Georgia, Athens, Georgia, 30602, USA., Crowley D; School of Chemical, Materials and Biomedical Engineering, College of Engineering, University of Georgia, Athens, Georgia, 30602, USA., Hill J; Department of Surgery, University of Michigan Medical Center, Ann Arbor, Michigan, 48109, USA., Morales Y; Department of Surgery, University of Michigan Medical Center, Ann Arbor, Michigan, 48109, USA., Bartlett R; Department of Surgery, University of Michigan Medical Center, Ann Arbor, Michigan, 48109, USA., Brisbois EJ; School of Chemical, Materials and Biomedical Engineering, College of Engineering, University of Georgia, Athens, Georgia, 30602, USA., Handa H; Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia, Athens, Georgia, 30602, USA.; School of Chemical, Materials and Biomedical Engineering, College of Engineering, University of Georgia, Athens, Georgia, 30602, USA.
Jazyk: angličtina
Zdroj: Advanced healthcare materials [Adv Healthc Mater] 2024 Sep; Vol. 13 (22), pp. e2400492. Date of Electronic Publication: 2024 Jul 03.
DOI: 10.1002/adhm.202400492
Abstrakt: Blood-contacting medical devices routinely fail from the cascading effects of biofouling toward infection and thrombosis. Nitric oxide (NO) is an integral part of endothelial homeostasis, maintaining platelet quiescence and facilitating oxidative/nitrosative stress against pathogens. Recently, it is shown that the surface evolution of NO can mediate cell-surface interactions. However, this technique alone cannot prevent the biofouling inherent in device failure with dynamic blood-contacting applications. This work proposes an endothelium-mimicking surface design pairing controlled NO release with an inherently antifouling polyethylene glycol interface (NO+PEG). This simple, robust, and scalable platform develops surface-localized NO availability with surface hydration, leading to a significant reduction in protein adsorption as well as bacteria/platelet adhesion. Further in vivo thrombogenicity studies show a decrease in thrombus formation on NO+PEG interfaces, with preservation of circulating platelet and white blood cell counts, maintenance of activated clotting time, and reduced coagulation cascade activation. It is anticipated that this bio-inspired surface design will enable a facile alternative to existing surface technologies to address clinical manifestations of infection and thrombosis in dynamic blood-contacting environments.
(© 2024 The Author(s). Advanced Healthcare Materials published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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