Engineered Probiotic Bio-Heterojunction with Robust Antibiofilm Modality via "Eating" Extracellular Polymeric Substances for Wound Regeneration.

Autor: Qin M; Research Center for Nano-Biomaterials & Regenerative Medicine, Department of Biomedical Engineering, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, 030024, China., Zhang X; Research Center for Nano-Biomaterials & Regenerative Medicine, Department of Biomedical Engineering, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, 030024, China., Ding H; School of Chemical Engineering, Sichuan University, Chengdu, 610065, China., Chen Y; School of Chemical Engineering, Sichuan University, Chengdu, 610065, China., He W; School of Chemical Engineering, Sichuan University, Chengdu, 610065, China., Wei Y; Research Center for Nano-Biomaterials & Regenerative Medicine, Department of Biomedical Engineering, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, 030024, China.; Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering, Taiyuan, 030060, China., Chen W; Research Center for Nano-Biomaterials & Regenerative Medicine, Department of Biomedical Engineering, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, 030024, China.; Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering, Taiyuan, 030060, China., Chan YK; Department of Mechanical Engineering, The University of Hong Kong, Hong Kong, 999077, China., Shi Y; NHC Key Laboratory of Pneumoconiosis, Shanxi Key Laboratory of Respiratory Diseases, Department of Pulmonary and Critical Care Medicine, The First Hospital of Shanxi Medical University, Taiyuan, 030001, China., Huang D; Research Center for Nano-Biomaterials & Regenerative Medicine, Department of Biomedical Engineering, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, 030024, China.; Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering, Taiyuan, 030060, China., Deng Y; School of Chemical Engineering, Sichuan University, Chengdu, 610065, China.; Department of Mechanical Engineering, The University of Hong Kong, Hong Kong, 999077, China.; State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu, 610065, China.
Jazyk: angličtina
Zdroj: Advanced materials (Deerfield Beach, Fla.) [Adv Mater] 2024 Aug; Vol. 36 (35), pp. e2402530. Date of Electronic Publication: 2024 Jul 01.
DOI: 10.1002/adma.202402530
Abstrakt: The compact three-dimensional (3D) structure of extracellular polymeric substances (EPS) within biofilms significantly hinders the penetration of antimicrobial agents, making biofilm eradication challenging and resulting in persistent biofilm-associated infections. To address this challenge, a solution is proposed: a probiotic bio-heterojunction (P-bioHJ) combining Lactobacillus rhamnosus with MXene (Ti 3 C 2 ) quantum dots (MQDs)/FeS heterojunction. This innovation aims to break down the saccharides in EPS, enabling effective combat against biofilm-associated infections. Initially, the P-bioHJ targets saccharides through metabolic processes, causing the collapse of EPS and allowing infiltration into bacterial colonies. Simultaneously, upon exposure to near-infrared (NIR) irradiation, the P-bioHJ produces reactive oxygen species (ROS) and thermal energy, deploying physical mechanisms to combat bacterial biofilms effectively. Following antibiofilm treatment, the P-bioHJ adjusts the oxidative environment, reduces wound inflammation by scavenging ROS, boosts antioxidant enzyme activity, and mitigates the NF-κB inflammatory pathway, thereby accelerating wound healing. In vitro and in vivo experiments confirm the exceptional antibiofilm, antioxidant/anti-inflammatory, and wound-regeneration properties of P-bioHJ. In conclusion, this study provides a promising approach for treating biofilm-related infections.
(© 2024 Wiley‐VCH GmbH.)
Databáze: MEDLINE
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