| Autor: |
Yang CH; Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea., Cho HS; Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea., Kim YH; Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea., Yoo K; Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea., Lim J; Nanophilia Inc., Gwacheon 13840, Republic of Korea., Hahm E; Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea., Rho WY; School of International Engineering and Science, Jeonbuk National University, Jeonju 54896, Republic of Korea., Kim YJ; Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea., Jun BH; Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea. |
| Abstrakt: |
Surface-enhanced Raman spectroscopy (SERS) tagging using silica(SiO 2 )@Ag nanoparticles (NPs) is easy to handle and is being studied in various fields, including SERS imaging and immunoassays. This is primarily due to its structural advantages, characterized by high SERS activity. However, the Ag NPs introduced onto the SiO 2 surface may undergo structural transformation owing to the Ostwald ripening phenomenon under various conditions. As a result, the consistency of the SERS signal decreases, reducing their usability as SERS substrates. Until recently, research has been actively conducted to improve the stability of single Ag NPs. However, research on SiO 2 @Ag NPs used as a SERS-tagging material is still lacking. In this study, we utilized a Raman labeling compound (RLC) to prevent the structural deformation of SiO 2 @Ag NPs under various conditions and proposed excellent SiO 2 @Ag@RLC-Pre NPs as a SERS-tagging material. Using various RLCs, we confirmed that 4-mercaptobenzoic acid (4-MBA) is the RLC that maintains the highest stability for 2 months. These results were also observed for the SiO 2 @Ag NPs, which were unstable under various pH and temperature conditions. We believe that SERS tags using SiO 2 @Ag NPs and 4-MBA can be utilized in various applications on based SERS because of the high stability and consistency of the resulting SERS signal. |
