Strain dynamics of contaminating bacteria modulate the yield of ethanol biorefineries.

Autor: de Oliveira Lino FS; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, 2800, Denmark., Garg S; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, 2800, Denmark., Li SS; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, 2800, Denmark.; School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, 4072, Australia., Misiakou MA; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, 2800, Denmark., Kang K; Leibniz Institute for Natural Product Research and Infection Biology, Jena, 07745, Germany., Vale da Costa BL; Escola de Engenharia de Alimentos da Universidade de Campinas, 13083-862, Campinas, SP, Brazil., Beyer-Pedersen TS; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, 2800, Denmark., Giacon TG; Departamento de Engenharia Química da Escola Politécnica da Universidade de São Paulo. Universidade de São Paulo, 05508-000, São Paulo, SP, Brazil., Basso TO; Departamento de Engenharia Química da Escola Politécnica da Universidade de São Paulo. Universidade de São Paulo, 05508-000, São Paulo, SP, Brazil., Panagiotou G; Leibniz Institute for Natural Product Research and Infection Biology, Jena, 07745, Germany., Sommer MOA; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, 2800, Denmark. msom@bio.dtu.dk.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Jun 22; Vol. 15 (1), pp. 5323. Date of Electronic Publication: 2024 Jun 22.
DOI: 10.1038/s41467-024-49683-2
Abstrakt: Bioethanol is a sustainable energy alternative and can contribute to global greenhouse-gas emission reductions by over 60%. Its industrial production faces various bottlenecks, including sub-optimal efficiency resulting from bacteria. Broad-spectrum removal of these contaminants results in negligible gains, suggesting that the process is shaped by ecological interactions within the microbial community. Here, we survey the microbiome across all process steps at two biorefineries, over three timepoints in a production season. Leveraging shotgun metagenomics and cultivation-based approaches, we identify beneficial bacteria and find improved outcome when yeast-to-bacteria ratios increase during fermentation. We provide a microbial gene catalogue which reveals bacteria-specific pathways associated with performance. We also show that Limosilactobacillus fermentum overgrowth lowers production, with one strain reducing yield by ~5% in laboratory fermentations, potentially due to its metabolite profile. Temperature is found to be a major driver for strain-level dynamics. Improved microbial management strategies could unlock environmental and economic gains in this US $ 60 billion industry enabling its wider adoption.
(© 2024. The Author(s).)
Databáze: MEDLINE
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