| Autor: |
Đukić V; Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia., Usman M; Fungal Biology and Systematics Research Laboratory, Institute of Botany, University of the Punjab, Lahore, Pakistan., Khalid AN; Fungal Biology and Systematics Research Laboratory, Institute of Botany, University of the Punjab, Lahore, Pakistan., Manojlović A; Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia., Zarić M; Department of Biochemistry, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia., Čanović P; Department of Biochemistry, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia., Živković-Zarić R; Department of Pharmacology and Toxicology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia., Manojlović N; Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia. |
| Abstrakt: |
For the first-time, chemical composition and in vitro antitumor activity was investigated of a newly described lichen Anamylopsora pakistanica Usman & Khalid from the second highest plateau of the world (Deosai Plains, Pakistan). HPLC-UV method was used for identification of secondary metabolites and the acetone extract had higher values of TPC (41.90 mg GA/g ) and TFC (75.37 mg RE/g) as compared to methanol extract. As chemical constituents 5,7-dihydroxy-6-methylphthalide, haematommic acid and alectorialic acid, were identified as major compounds. Atranol, alectorialin, gyrophoric acid and usnic acid were detected as minor substances. Acetone and methanol extracts induced a dose-dependent and time-dependent decrease in the viability of three types of tumour cells HeLa, HCT116 and MDA-MB-231. This lichen extract can induce S phase arrest in HeLa as compared to the untreated cells. Extract of this unique lichen, A. pakistanica, can be used safely as a significant source of biologically active compounds. |
