Tear Cytokines as Predictive Biomarkers of Success in Contact Lens Discomfort Management.

Autor: Calderón-García AÁ; Institute of Applied Ophthalmobiology (IOBA), Universidad de Valladolid, Valladolid, Spain.; Universidad Católica de Ávila, Ávlia, Spain., López-de la Rosa A; Institute of Applied Ophthalmobiology (IOBA), Universidad de Valladolid, Valladolid, Spain.; Department of Theoretical Physics, Atomic and Optics, Universidad de Valladolid, Valladolid, Spain., Valencia-Nieto L; Institute of Applied Ophthalmobiology (IOBA), Universidad de Valladolid, Valladolid, Spain.; Department of Theoretical Physics, Atomic and Optics, Universidad de Valladolid, Valladolid, Spain., García-Vázquez C; Institute of Applied Ophthalmobiology (IOBA), Universidad de Valladolid, Valladolid, Spain., Arroyo-Del-Arroyo C; Institute of Applied Ophthalmobiology (IOBA), Universidad de Valladolid, Valladolid, Spain., López-Miguel A; Institute of Applied Ophthalmobiology (IOBA), Universidad de Valladolid, Valladolid, Spain., Blanco-Vázquez M; Institute of Applied Ophthalmobiology (IOBA), Universidad de Valladolid, Valladolid, Spain.; Department of Theoretical Physics, Atomic and Optics, Universidad de Valladolid, Valladolid, Spain., Novo-Diez A; Institute of Applied Ophthalmobiology (IOBA), Universidad de Valladolid, Valladolid, Spain.; Department of Theoretical Physics, Atomic and Optics, Universidad de Valladolid, Valladolid, Spain., Fernández I; Institute of Applied Ophthalmobiology (IOBA), Universidad de Valladolid, Valladolid, Spain.; Networking Research Center on Bioengineering Biomaterials and Nanomedicine (CIBER-BBN), Valladolid, Spain., Enríquez de Salamanca A; Institute of Applied Ophthalmobiology (IOBA), Universidad de Valladolid, Valladolid, Spain.; Networking Research Center on Bioengineering Biomaterials and Nanomedicine (CIBER-BBN), Valladolid, Spain., González-García MJ; Institute of Applied Ophthalmobiology (IOBA), Universidad de Valladolid, Valladolid, Spain.; Department of Theoretical Physics, Atomic and Optics, Universidad de Valladolid, Valladolid, Spain.; Networking Research Center on Bioengineering Biomaterials and Nanomedicine (CIBER-BBN), Valladolid, Spain.
Jazyk: angličtina
Zdroj: Ocular immunology and inflammation [Ocul Immunol Inflamm] 2024 Dec; Vol. 32 (10), pp. 2339-2347. Date of Electronic Publication: 2024 Jun 18.
DOI: 10.1080/09273948.2024.2361114
Abstrakt: Purpose: To analyze changes in tear levels of inflammatory mediators in symptomatic contact lens (CL) wearers after refitting with daily disposable CLs and to identify potential biomarkers of success in CL discomfort (CLD) management.
Methods: Symptomatic CL wearers (CLDEQ-8 ≥ 12) were refitted (V1) with daily disposable CLs (Delefilcon A). After one month (V2), participants were classified into the post-fitting non-symptomatic (CLDEQ <12) and symptomatic (CLDEQ ≥12) groups. At each visit, the participants were clinically evaluated, tears were collected, and 20 inflammatory mediators and substance P (SP) were measured using multiplex immunobead analysis and ELISA, respectively. The detection rates and concentrations were compared between visits and groups, and logistic regression models were performed.
Results: Forty-three subjects (32 women/11 men; mean age: 23.2 ± 4.9 years) were enrolled. The IL-1β and IL-9 detection rates were higher at V2 ( p  ≤ 0.044). The detection rates of IL-1β, IL-9, MIP-1α/CCL3, and MMP-9 at V1 ( p  ≤ 0.045) and IL-17A at V2 ( p  ≤ 0.014) were higher in the post-fitting symptomatic group. The tear IL-9 concentration was increased at V2 ( p  = 0.018). The tear concentrations of fractalkine/CX3CL1, IL-2, IL-6, IL-10, MCP-3/CCL7, MIP-1β, NGF, RANTES/CCL5, and TNF-α were higher in the post-fitting symptomatic group ( p  ≤ 0.044). Additionally, levels of fractalkine/CX3CL1, IL-2, IL-6, IL-10, RANTES/CCL5, and TNF-α at V1 were significantly associated with the post-fitting grouping ( p  ≤ 0.044).
Conclusions: Low tear concentrations of specific inflammatory mediators may be used as a predictive biomarker of success for refitting symptomatic CL wearers with daily disposable CLs. However, complementary treatments might be required for symptomatic CL wearers with higher levels of these inflammatory molecules.
Databáze: MEDLINE
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