Structural and biophysical insights into targeting of claudin-4 by a synthetic antibody fragment.

Autor: Erramilli SK; Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL, 60637, USA., Dominik PK; Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL, 60637, USA.; Pfizer, San Diego, CA, 92121, USA., Ogbu CP; Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.; Department of Structural Biology, University at Buffalo, Buffalo, NY, 14203, USA., Kossiakoff AA; Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL, 60637, USA., Vecchio AJ; Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA. vecchioa@buffalo.edu.; Department of Structural Biology, University at Buffalo, Buffalo, NY, 14203, USA. vecchioa@buffalo.edu.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2024 Jun 17; Vol. 7 (1), pp. 733. Date of Electronic Publication: 2024 Jun 17.
DOI: 10.1038/s42003-024-06437-6
Abstrakt: Claudins are a 27-member family of ~25 kDa membrane proteins that integrate into tight junctions to form molecular barriers at the paracellular spaces between endothelial and epithelial cells. As the backbone of tight junction structure and function, claudins are attractive targets for modulating tissue permeability to deliver drugs or treat disease. However, structures of claudins are limited due to their small sizes and physicochemical properties-these traits also make therapy development a challenge. Here we report the development of a synthetic antibody fragment (sFab) that binds human claudin-4 and the determination of a high-resolution structure of it bound to claudin-4/enterotoxin complexes using cryogenic electron microscopy. Structural and biophysical results reveal this sFabs mechanism of select binding to human claudin-4 over other homologous claudins and establish the ability of sFabs to bind hard-to-target claudins to probe tight junction structure and function. The findings provide a framework for tight junction modulation by sFabs for tissue-selective therapies.
(© 2024. The Author(s).)
Databáze: MEDLINE
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