Self-assembled protein vesicles as vaccine delivery platform to enhance antigen-specific immune responses.

Autor: Li Y; BioEngineering Program, Georgia Institute of Technology, USA., Rodriguez-Otero MR; BioEngineering Program, Georgia Institute of Technology, USA; School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, USA., Champion JA; BioEngineering Program, Georgia Institute of Technology, USA; School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, USA. Electronic address: julie.champion@chbe.gatech.edu.
Jazyk: angličtina
Zdroj: Biomaterials [Biomaterials] 2024 Dec; Vol. 311, pp. 122666. Date of Electronic Publication: 2024 Jun 12.
DOI: 10.1016/j.biomaterials.2024.122666
Abstrakt: Self-assembling protein nanoparticles are beneficial platforms for enhancing the often weak and short-lived immune responses elicited by subunit vaccines. Their benefits include multivalency, similar sizes as pathogens and control of antigen orientation. Previously, the design, preparation, and characterization of self-assembling protein vesicles presenting fluorescent proteins and enzymes on the outer vesicle surface have been reported. Here, a full-size model antigen protein, ovalbumin (OVA), was genetically fused to the recombinant vesicle building blocks and incorporated into protein vesicles via self-assembly. Characterization of OVA protein vesicles showed room temperature stability and tunable size. Immunization of mice with OVA protein vesicles induced strong antigen-specific humoral and cellular immune responses. This work demonstrates the potential of protein vesicles as a modular platform for delivering full-size antigen proteins that can be extended to pathogen antigens to induce antigen specific immune responses.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Julie A. Champion and Mariela R. Rodriguez-Otero report financial support was provided by National Science Foundation.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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