Investigation of SCCmec types using the real time PCR method in cefoxitin-resistant Staphylococcus aureus isolates.

Autor: Sağlam M; Gaziantep University Institute of Natural and Applied Sciences, Department of Biology, 27310 Şehitkamil/Gaziantep, Turkey. Electronic address: msaglam_27@hotmail.com., Kılıç İH; Gaziantep University Faculty of Arts and Science, Department of Biology, Gaziantep, Turkey. Electronic address: kilic@gantep.edu.tr., Zer Y; Gaziantep University Faculty of Medicine Department of Medical Microbiology, Gaziantep, Turkey. Electronic address: yaseminzer@hotmail.com.
Jazyk: angličtina
Zdroj: Indian journal of medical microbiology [Indian J Med Microbiol] 2024 Jul-Aug; Vol. 50, pp. 100649. Date of Electronic Publication: 2024 Jun 14.
DOI: 10.1016/j.ijmmb.2024.100649
Abstrakt: Background: Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen that can cause many community and hospital-acquired infections. This study was conducted to investigate the SCCmec gene types responsible for methicillin resistance in MRSA isolates isolated from hospitalised patients.
Material and Methods: MRSA isolates isolated from samples sent from various clinics to Gaziantep University Hospital Microbiology Laboratory between March 2021-January 2022 were included in the study. Bacteria were identified using by VITEK 2 automated system. Cefoxitin (FOX) resistance was determined by the disc diffusion method according to EUCAST standards. Cefoxitin resistance was confirmed by the Penicillin Binding Protein 2' latex agglutination test. Types of mecA, mecC, coa, nuc, Panton Valentin Leukocidin (PVL), ccrC2, class A mec, SCCmec types in isolates detected as MRSA were investigated by real-time PCR.
Results: In this study, 116 isolates meeting the study criteria were examined. By detecting the nuc and coa genes in all isolates by PCR, the phenotypic identification of S.aureus was confirmed. While the mecA gene was detected in all MRSA isolates, no mecC gene was detected in any isolates. Detected SCCmec types were as follows; SCCmec Type 1 (2.6%), Type II (28.4%), Type III (12.9%), Type IVa (11.2%), Type IVb (3.4%), Type IVc (3.4%), Type IVg (12.1%), Type V (0.9%), Type VII (4.3%), Type VIII (18.1%), Type IX (0.9%), Type XII (1.7%). On the other hand, SCCmec Type VI, X, XI and XIII were not found in any isolate. It was determined that four of the MRSA isolates (3.4%) carried the PVL gene that two (50%) of these were found in SCCmec Type VIII.
Conclusion: Monitoring of FOX resistance is an effective and safe method for determination of MRSA isolates. The change in the mec gene causes resistance, which should be monitored regularly with molecular methods. Our study is the first study in Turkey.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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