Hydrazone-flavonol based oxidovanadium(V) complexes: Synthesis, characterization and antihyperglycemic activity of chloro derivative in vivo.

Autor: Zahirović A; Laboratory for Inorganic and Bioinorganic Chemistry, Department of Chemistry, Faculty of Science, University of Sarajevo, Sarajevo, Bosnia and Herzegovina. Electronic address: adnan.zahirovic@pmf.unsa.ba., Fočak M; Department of Biology, Faculty of Science, University of Sarajevo, Sarajevo, Bosnia and Herzegovina., Fetahović S; Laboratory for Inorganic and Bioinorganic Chemistry, Department of Chemistry, Faculty of Science, University of Sarajevo, Sarajevo, Bosnia and Herzegovina., Tüzün B; Plant and Animal Production Department, Technical Sciences Vocational School of Sivas, Sivas Cumhuriyet University, Sivas, Turkey., Višnjevac A; Laboratory for Chemical and Biological Crystallography, Division of Physical Chemistry, Ruđer Bošković Institute, Zagreb, Croatia., Muzika V; Department of Histology and Embryology, Faculty of Medicine, University of Sarajevo, Bosnia and Herzegovina., Brulić MM; Department of Biology, Faculty of Science, University of Sarajevo, Sarajevo, Bosnia and Herzegovina., Žero S; Laboratory for Inorganic and Bioinorganic Chemistry, Department of Chemistry, Faculty of Science, University of Sarajevo, Sarajevo, Bosnia and Herzegovina., Čustović S; Department of Histology and Embryology, Faculty of Medicine, University of Sarajevo, Bosnia and Herzegovina., Crans DC; Cell & Molecular Biology Program, Colorado State University, Fort Collins, USA., Roca S; NMR Centre, Ruđer Bošković Institute, Zagreb, Croatia. Electronic address: suncica.roca@irb.hr.
Jazyk: angličtina
Zdroj: Journal of inorganic biochemistry [J Inorg Biochem] 2024 Sep; Vol. 258, pp. 112637. Date of Electronic Publication: 2024 Jun 06.
DOI: 10.1016/j.jinorgbio.2024.112637
Abstrakt: Wet synthesis approach afforded four new heteroleptic mononuclear neutral diamagnetic oxidovanadium(V) complexes, comprising salicylaldehyde-based 2-furoic acid hydrazones and a flavonol coligand of the general composition [VO(fla)(L-ONO)]. The complexes were comprehensively characterized, including chemical analysis, conductometry, infrared, electronic, and mass spectroscopy, as well as 1D 1 H and proton-decoupled 13 C( 1 H) NMR spectroscopy, alongside extensive 2D 1 H 1 H COSY, 1 H 13 C HMQC, and 1 H 13 C HMBC NMR analyses. Additionally, the quantum chemical properties of the complexes were studied using Gaussian at the B3LYP, HF, and M062X levels on the 6-31++g(d,p) basis sets. The interaction of these hydrolytically inert vanadium complexes and the BSA was investigated through spectrofluorimetric titration, synchronous fluorimetry, and FRET analysis in a temperature-dependent manner, providing valuable thermodynamic insights into van der Waals interactions and hydrogen bonding. Molecular docking was conducted to gain further understanding of the specific binding sites of the complexes to BSA. Complex 2, featuring a 5-chloro-substituted salicylaldehyde component of the hydrazone, was extensively examined for its biological activity in vivo. The effects of complex administration on biochemical and hematological parameters were evaluated in both healthy and diabetic Wistar rats, revealing antihyperglycemic activity at millimolar concentration. Furthermore, histopathological analysis and bioaccumulation studies of the complex in the brain, kidneys, and livers of healthy and diabetic rats revealed the potential for further development of vanadium(V) hydrazone complexes as antidiabetic and insulin-mimetic agents.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023. Published by Elsevier Inc.)
Databáze: MEDLINE
Externí odkaz: