Transcription factors BZR1 and PAP1 cooperate to promote anthocyanin biosynthesis in Arabidopsis shoots.

Autor:	Lee SH; Department of Life Science, Hanyang University, Seoul 04763, Republic of Korea.; Research Institute for Convergence of Basic Science, Hanyang University, Seoul 04763, Republic of Korea., Kim SH; Department of Life Science, Hanyang University, Seoul 04763, Republic of Korea.; Research Institute for Convergence of Basic Science, Hanyang University, Seoul 04763, Republic of Korea., Park TK; Department of Life Science, Hanyang University, Seoul 04763, Republic of Korea.; Hanyang Institute of Bioscience and Biotechnology, Hanyang University, Seoul 04763, Republic of Korea., Kim YP; Department of Life Science, Hanyang University, Seoul 04763, Republic of Korea.; Research Institute for Convergence of Basic Science, Hanyang University, Seoul 04763, Republic of Korea.; Hanyang Institute of Bioscience and Biotechnology, Hanyang University, Seoul 04763, Republic of Korea., Lee JW; Department of Life Science, Hanyang University, Seoul 04763, Republic of Korea.; Research Institute for Convergence of Basic Science, Hanyang University, Seoul 04763, Republic of Korea.; Hanyang Institute of Bioscience and Biotechnology, Hanyang University, Seoul 04763, Republic of Korea., Kim TW; Department of Life Science, Hanyang University, Seoul 04763, Republic of Korea.; Research Institute for Convergence of Basic Science, Hanyang University, Seoul 04763, Republic of Korea.; Hanyang Institute of Bioscience and Biotechnology, Hanyang University, Seoul 04763, Republic of Korea.
Jazyk:	angličtina
Zdroj:	The Plant cell [Plant Cell] 2024 Sep 03; Vol. 36 (9), pp. 3654-3673.
DOI:	10.1093/plcell/koae172
Abstrakt:	Anthocyanins play critical roles in protecting plant tissues against diverse stresses. The complicated regulatory networks induced by various environmental factors modulate the homeostatic level of anthocyanins. Here, we show that anthocyanin accumulation is induced by brassinosteroids (BRs) in Arabidopsis (Arabidopsis thaliana) shoots and shed light on the underlying regulatory mechanism. We observed that anthocyanin levels are altered considerably in BR-related mutants, and BRs induce anthocyanin accumulation by upregulating the expression of anthocyanin biosynthetic genes. Our genetic analysis indicated that BRASSINAZOLE RESISTANT 1 (BZR1) and PRODUCTION OF ANTHOCYANIN PIGMENT 1 (PAP1) are essential for BR-induced anthocyanin accumulation. The BR-responsive transcription factor BZR1 directly binds to the PAP1 promoter, regulating its expression. In addition, we found that intense anthocyanin accumulation caused by the pap1-D-dominant mutation is significantly reduced in BR mutants, implying that BR activity is required for PAP1 function after PAP1 transcription. Moreover, we demonstrated that BZR1 physically interacts with PAP1 to cooperatively regulate the expression of PAP1-target genes, such as TRANSPARENT TESTA 8, DIHYDROFLAVONOL 4-REDUCTASE, and LEUKOANTHOCYANIDIN DIOXYGENASE. Our findings indicate that BZR1 functions as an integral component of the PAP1-containing transcription factor complex, contributing to increased anthocyanin biosynthesis. Notably, we also show that functional interaction of BZR1 with PAP1 is required for anthocyanin accumulation induced by low nitrogen stress. Taken together, our results demonstrate that BR-regulated BZR1 promotes anthocyanin biosynthesis through cooperative interaction with PAP1 of the MBW complex. Competing Interests: Conflict of interest statement. The authors declare no confilct of interests. (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)
Databáze:	MEDLINE
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