Characterization of resistance and virulence factors in livestock-associated methicillin-resistant Staphylococcus aureus.

Autor: Beshiru A; Applied Microbial Processes & Environmental Health Research Group, Faculty of Life Sciences, University of Benin, PMB 1154, Benin City, 300283, Nigeria.; Department of Microbiology, College of Natural and Applied Sciences, Western Delta University, Oghara, Nigeria., Igbinosa IH; Applied Microbial Processes & Environmental Health Research Group, Faculty of Life Sciences, University of Benin, PMB 1154, Benin City, 300283, Nigeria.; Department of Environmental Management & Toxicology, Faculty of Life Sciences, University of Benin, PMB 1154, Benin City, 300283, Nigeria., Akinnibosun O; Applied Microbial Processes & Environmental Health Research Group, Faculty of Life Sciences, University of Benin, PMB 1154, Benin City, 300283, Nigeria.; Department of Microbiology, Faculty of Science, Federal University of Health Sciences, PMB 145, Otukpo, 927101, Otukpo, Nigeria., Ogofure AG; Applied Microbial Processes & Environmental Health Research Group, Faculty of Life Sciences, University of Benin, PMB 1154, Benin City, 300283, Nigeria., Dunkwu-Okafor A; Applied Microbial Processes & Environmental Health Research Group, Faculty of Life Sciences, University of Benin, PMB 1154, Benin City, 300283, Nigeria., Uwhuba KE; Department of Microbiology, College of Natural and Applied Sciences, Western Delta University, Oghara, Nigeria., Igbinosa EO; Applied Microbial Processes & Environmental Health Research Group, Faculty of Life Sciences, University of Benin, PMB 1154, Benin City, 300283, Nigeria. Etinosa.Igbinosa@uniben.edu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Jun 09; Vol. 14 (1), pp. 13235. Date of Electronic Publication: 2024 Jun 09.
DOI: 10.1038/s41598-024-63963-3
Abstrakt: The study investigated the economic concerns associated with livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) in livestock (cow), examining its connection to severe infections, antimicrobial resistance (AMR), and virulence factors. The research, conducted in Edo State, Nigeria, analyzed 400 samples (200 rectal and 200 nasal swabs) collected between March 2018 and February 2019. MRSA prevalence was identified using conventional culture-based methods and polymerase chain reaction (PCR) techniques, revealing 63.5% (n = 254) for Staphylococcus aureus and 55% (n = 220) for MRSA. Of the 76 mecA-positive MRSA isolates, 64.5% (n = 49) exhibited multidrug resistance (MDR) while the remaining were sensitive to specific antimicrobials. Key virulence genes, such as PVL (81.6%; n = 62) and tsst-1 (44.7%; n = 34), were prevalent, along with AMR genes like mecC, tetM, ermA, ermC, vanA, and vanC. Staphylococcal chromosomal cassette mec (SCCmec) typing identified different types, notably II, IVa, and IVb. Biofilm formation, a crucial virulence factor varied in strength, is associated with icaA and icaB genes (p < 0.01). The findings highlighted substantial AMR and biofilm-forming capacity within LA-MRSA isolates, emphasizing the importance of ongoing surveillance for informed treatment strategies, AMR policies, and control measures against MDR staphylococcal infections.
(© 2024. The Author(s).)
Databáze: MEDLINE
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